Sequences from confirmation amplicon


Confirmation sequences for line 054H07 and hit At2g17370 are shown.
Line ID 054H07
Locus At2g17370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7549669
Primers used for PCR 02hk - 8474, show primer details
   
Sequence Name 26-K112562-0022-054-H07C-02hk
GenBank Accession LN497999 [GenBank]
Sequencing primer gene specific primer, 02hk: 5'-AGGTAGATAAACGAAGCAACGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K112562-0022-054-H07C-02hk
TAGTCACGACGTGAAGAGGCGTCGAGTTACGGATCTTCTCACGCCATCGGCTAAGAAGAA
AATAAACAGTAGCGAAGAAGAGCGAGAGGAAAAAAGTGTTGGTTAGGTATAACGGAAGAG
GAAGCGCGTCAGAGGCTTTACGAAGAGGAGGATCGACGGCGACATTAGAGATTTCTTCTC
CGTTCTTTTTAGTCGGAAATCTACGACGGAGATCCTCCATTGACGCCGGAGAGAAGGAGG
AGATTTGTTTTTAATGGTTATGAGATTATTTTGGGCTCCAAAGTCCAAAGTGTGAGATGA
CAAAAATAGGTGAAGAGTGTTATTTATAGTGTTGTGTGTGACATCGAAATCAACGTCAAG
GTTGTTGGGTCATCATCGTATGTGAGAGAGGAGAAGAAGAAGAACAGAGATGATGATTTT
GTCTCCTCCGTCACAATCTAACTGCTTCTTTTATTATTTCCACTTTTGGGATGATTATTT
AGGGTTATGGTTTTTGTTACTTTTGATTCTTTTTTTCAACACATTTAATGTTATGGATTT
GATTGTTACTACTTACTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAC
   
Sequence Name 02-K112561-0022-054-H07C-8409
GenBank Accession LN487103 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K112561-0022-054-H07C-8409
ATTTAGTAGTAGTAACATCAAATCCATAACATTAAATGTGTTGAAAAAAAGAATCAAAAG
TAACAAAAACCATAACCCTAAATAATCATCCCAAAAGTGGAAATAATAAAAGAAGCAGTT
AGATTGTGACGGAGGAGACAAAATCATCATCTCTGTTCTTCTTCTTCTCCTCTCTCACAT
ACGATGATGACCCAACAACCTTGACGTTGATTTCGATGTCACACACAACACTATAAATAA
CACTCTTCACCTATTTTTGTCATCTCACACTTTGGACTTTGGAGCCCAAAATAATCTCAT
AACCATTAAAAACAAATCTCCTCCTTCTCTCCGGCGTCAATGGAGGATCTCCGTCGTAGA
TTTCCGACTAAAAAGAACGGAGAAGAAATCTCTAATGTCGCCGTCGATCCTCCTCTTCGT
AAAGCCTCTGACGCGCTTCCTCTTCCGTTATACCTAACCAACACTTTTTTCCTCTCGCTC
TTCTTCGCTACTGTTTATTTTCTTCTTAGCCGATGGCGTGAGAAGATCCGTAACTCGACG
CCTCTTCACGTCGTTGACTTATCTGAGATCTGTGCTCTAATCGGTTTCGTTGCTTCGTTA
ATTCTA

Last Updated on 06.12.2018 16:11