Sequences from confirmation amplicon


Confirmation sequences for line 055A02 and hit At5g56600 are shown.
Line ID 055A02
Locus At5g56600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22910636
Primers used for PCR IN01 - 8474, show primer details
   
Sequence Name 56-K016269-022-055-A02C-8409
GenBank Accession LN508959 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K016269-022-055-A02C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCAACAATTTTCC
TCAGGTTAGGGTTTATTTCTATACCGACTACTCAATTCTTATCATCTTGCTGTTTCTGGT
TTGGTTCTATGTTGAAATCTGCAACCTTTGGTTTTGTTTAGCTACTAAACCACCAAAATT
CAGCAATTTTCAATGTTTGGTTTTGATTTTCTGATTCATATGTTCTTGAGGTTATAGAGT
GCAAAAGATGCATTAGAAGAAGTTCGAATCACAAGTTTAAAGCTCTTTTAAGAAGAAAGT
TTTGACCTTTGGAAATAGACAAGTTCTTTGTACTGGTTATGTTGTTCATAAGTTATTAAG
TTGCTTTCATGAGATGTTTAAGAGAGACTGAATTTGTTTCTCTTATAGTTTTTGTATTGT
TGTTGTTTTAGGTGAAGCCTGAGGAAATTCAGGGCATCAAAGACGATTTTACTACACCTG
GAACACTTGCCCCAACAGGATTGTTCCTTGG
   
Sequence Name 60-K016269-022-055-A02C-IN01
GenBank Accession LN510122 [GenBank]
Sequencing primer gene specific primer, IN01: 5'-ACTTGTTACCACCAAGGAACAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K016269-022-055-A02C-in01
TGGGGCAAGTGTTCCAGGTGTAGTAAAATCGTCTTTGATGCCCTGAATTTCCTCAGGCTT
CACCTAAAACAACAACAATACAAAAACTATAAGAGAAACAAATTCAGTCTCTCTTAAACA
TCTCATGAAAGCAACTTAATAACTTATGAACAACATAACCAGTACAAAGAACTTGTCTAT
TTCCAAAGGTCAAAACTTTCTTCTTAAAAGAGCTTTAAACTTGTGATTCGAACTTCTTCT
AATGCATCTTTTGCACTCTATAACCTCAAGAACATATGAATCAGAAAATCAAAACCAAAC
ATTGAAAATTGCTGAATTTTGGTGGTTTAGTAGCTAAACAAAACCAAAGGTTGCAGATTT
CAACATAGAACCAAACCAGAAACAGCAAGATGATAAGAATTGAGTAGTCGGTATAGAAAT
AAACCCTAACCTGAGGAAAATTGTTGCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTT
Primers used for PCR FM26 - 8474, show primer details
   
Sequence Name 41-K029300-022-055-A02C-8409
GenBank Accession LN503957 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K029300-022-055-A02C-8409
CCATTTACAATTGCGGCGGCGGTGAGGCGGTTGCCTGCAACATCGCACATTAAGTGATCG
TCGACGTAAGTCTGCCACGACATGTTTGTTTTCTTCTTCTTCTTTACTTTTGCTCTGCTT
CTTCTTTGTGGTTTATCGGAATGTTCTAGAGATAGCGTCGAAACTACGAGTGAGTGAGTG
TGTGGCAATGGCATTTACGCAAATATATTGTTTTATACATGATTGACACGTGCCAGACAC
AGCTCTTTTTAATAGTTTCTAAAGAGGAAAATTGTATTAGAAAATCAACCAGTTATAATG
AAAAGGGAGGAAATAAATAAAAAAATAGAAAACTTATTTAAGGAAAACCAAAACATTTAA
TTAAATATGGTCTTTTCCTAAAAAAAAACAAATGTTTCGATTTTCATTATAATTGATTTT
TGTTATGAATAAAGATGGATGTCCATTATAAGGCCCTCATCTAAACCCATCATCTAGATG
TTTTTAGGGTTTTATCTTTTTTTTGTACTCCTATATAAAGAACCATTTATCTCAATGGAA
TAACACACTCATTTCTTTTATCTCTTTCTCTGGTTATAACACGTTATC
   
Sequence Name 42-K029300-022-055-A02C-FM26
GenBank Accession LN504340 [GenBank]
Sequencing primer gene specific primer, FM26: 5'-CTTTTAGAACGTGCTGATAACGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K029300-022-055-A02C-FM26
AATGAAGTGTGTATTCCTTGAGATAAATGGTTCTTTATATAGGAGTACAAAAAAAAGATA
AAACCCTAAAAACATCTAGATGATGGGTTTAGATGAGGGCCTTATAATGGACATCCATCT
TTATTCATAACAAAAATCAATTATAATGAAAATCGAAACATTTGTTTTTTTTTAGGAAAA
GACCATATTTAATTAAATGTTTTGGTTTTCCTTAAATAAGTTTTCTATTTTTTTATTTAT
TTCCTCCCTTTTCATTATAACTGGTTGATTTTCTAATACAATTTTCCTCTTTAGAAACTA
TTAAAAAGAGCTGTGTCTGGCACGTGTCAATCATGTATAAAACAATATATTTGCGTAAAT
GCCATTGCCACACACTCACTCACTCGTAGTTTCGACGCTATCTCTAGAACATTCCGATAA
ACCACAAAGAAGAAGCAGAGCAAAAGTAAAGAAGAAGAAGAAAACAAACATGTCGTGGCA
GACTTACGTCGACGATCACTTAATGTGCGATGTTGCAGGCAACCGCCTCACCGCCGCCGC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTA
TTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTC
T

Last Updated on 06.12.2018 16:11