Sequences from confirmation amplicon


Confirmation sequences for line 055F04 and hit At1g08450 are shown.
Line ID 055F04
Locus At1g08450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2671824
Primers used for PCR SP10 - 8474, show primer details
   
Sequence Name 61-K103548-0022-055-F04C-SP10
GenBank Accession LN510496 [GenBank]
Sequencing primer gene specific primer, SP10: 5'-TTCTGAAAATCAAGTGAGACCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K103548-0022-055-F04C-SP10
GGTGGGCGAAAGGAAGTACCTTTATTGTCAGGATCGCCGGGCCATTTACCGGCAGTGTGC
TTAAAGGTTCCAGCTTTGCCTTCGTTTCTTTTCCAATCAGACAAGACCCACCTACTTTTC
CATCCACCTGAACACAATCTTACTAAGTTAATTCCATAAACCTATAAATAGAAAGCCAAA
ATCCAGAACCATAGAATCGTTATATTAACCAGAATAGGAAAAGCAAAGCCTATAATAGAA
GATTCTAGTGAAGGATGGATTCGGATTCACTATCCTAATCAAATCAAAATCTACCAAGGA
AAGTTACTACTTACTAATCATAGCAATATATTCAAAAAGGAAACAAAAATAACAAATCGT
TGTTTTGTTTCCCAGAAAAGGAAAAAAAAGAGAGAAGAGTGTTTATATATATACTTCACC
TTCGAAATGCTCCTCTAAGAAGATCTCGGAGAAAGCCAATGGGGCAAGAGTTAGTACAGA
GACGAGGAAGAAGAAGCAGAAAAAACTGAGCTTATTTTGAGGTAATCCCATTTTTCAGAA
TTTCTCACTTCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 45-K103549-0022-055-F04C-8409
GenBank Accession LN505502 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K103549-0022-055-F04C-8409
GCCATTTACAATTGAATATATCCTGATGAAGTGAGAAATTCTGAAAAATGGGATTACCTC
AAAATAAGCTCAGTTTTTTCTGCTTCTTCTTCCTCGTCTCTGTACTAACTCTTGCCCCAT
TGGCTTTCTCCGAGATCTTCTTAGAGGAGCATTTCGAAGGTGAAGTATATATATAAACAC
TCTTCTCTCTTTTTTTTCCTTTTCTGGGAAACAAAACAACGATTTGTTATTTTTGTTTCC
TTTTTGAATATATTGCTATGATTAGTAAGTAGTAACTTTCCTTGGTAGATTTTGATTTGA
TTAGGATAGTGAATCCGAATCCATCCTTCACTAGAATCTTCTATTATAGGCTTTGCTTTT
CCTATTCTGGTTAATATAACGATTCTATGGTTCTGGATTTTGGCTTTCTATTTATAGGTT
TATGGAATTAACTTAGTAAGATTGTGTTCAGGTGGATGGAAAAGTAGGTGGGTCTTGTCT
GATTGGAAAAGAAACGAAGGCAAAGCTGGAACCTTTAAGCACACTGCCGGTAAATGGCCC
GGCGATCCTGACAATAAAGGTACTTCCTTTCGCCCACCTTTAAGCACACTGCAGAACTCC
GTCTTTTTGGCGTTAAGGGTCTCACTTGATTTTTC

Last Updated on 06.12.2018 16:11