Sequences from confirmation amplicon


Confirmation sequences for line 055H05 and hit At5g15320 are shown.
Line ID 055H05
Locus At5g15320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4977259
Primers used for PCR 57wv - 8474, show primer details
   
Sequence Name 62-K118656-0022-055-H05C-57wv
GenBank Accession LN510861 [GenBank]
Sequencing primer gene specific primer, 57wv: 5'-TGTTTTGCTTGCTATTTGTTTACG-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K118656-0022-055-H05C-57wv
TGCAAGATAAAAGAAATTCCAACAAAACTTGTTTAGTCGTCTATTTCTAGAGATTCAAAG
TGTGTTTCTATATTTCTTGTAGTATTATTTTGCTCCGAGATGCTTTAAGTTAAAATAACA
TAAAAACATGCATGAGCACATCATTTCATGGACGTCTTTCGCCATGAATAGGTTGCTGCA
AAGCATTGGTCTTCGGCAACTTGAAGACATTTTTGGTCATTCTTGAGTGACCGAATCGTA
TCTTTTTGATTCATTAAGATCCAAGATATTGAATGTTCTCTGACGAACACGATTACTACA
TATCAACCGTCCCCCGGATTAAAAAAATCTATCTTGAACACAGTTTTTGTACGTTACCAC
TTCTACAAATTAAACCAAAATCACTCTACTAATTAACCAGTAACCACTACACTAGAAAGT
ATACATATACTGTGGTCAATTTTATTTTATTTTATTTTGTTAACTACGTAAGAAATGTGA
AAGATTGAGAGTTACATGATGATAATGTGGATGTGATAAAAACAACACGTTGACATGTCG
ATTACTATATGAGAAAATTAAATGTTGGTTGTTTCATGTCCTTTTCTGCCTTGGGCTTTG
CCTCTCTCTCTCGAATCCGATTTTTCATTTTTTTCATATTAATTGTGAAAATAATATAAG
AAACACAAAAGCCGAAAAAAAAGACCAAGCCAATATTTCATAATGAAATAATAAATATGA
ATCGTAGTGATAAATAGGATACTATATTTATTTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGT
   
Sequence Name 36-K118655-0022-055-H05C-8409
GenBank Accession LN502412 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K118655-0022-055-H05C-8409
GCCATTTACAAATAAATATAGTATCCTATTTATCACTACGATTCATATTTATTATTTCAT
TATGAAATATTGGCTTGGTCTTTTTTTTCGGCTTTTGTGTTTCTTATATTATTTTCACAA
TTAATATGAAAAAAATGAAAAATCGGATTCGAGAGAGAGAGGCAAAGCCCAAGGCAGAAA
AGGACATGAAACAACCAACATTTAATTTTCTCATATAGTAATCGACATGTCAACGTGTTG
TTTTTATCACATCCACATTATCATCATGTAACTCTCAATCTTTCACATTTCTTACGTAGT
TAACAAAATAAAATAAAATAAAATTGACCACAGTATATGTATACTTTCTAGTGTAGTGGT
TACTGGTTAATTAGTAGAGTGATTTTGGTTTAATTTGTAGAAGTGGTAACGTACAAAAAC
TGTGTTCAAGATAGATTTTTTTAATCCGGGGGACGGTTGATATGTAGTAATCGTGTTCGT
CAGAGAACATTCAATATCTTGGATCTTAATGAATCAAAAAGATACGATTCGGTCACTCAA
GAATGACCAAAAATGTCTTCAAGTTGCCGAAGACCAATGCTTTGCAGCAACCTATTCATG
GCGAAAGACGTCCATGAAATGATGTGCTCATGCATGTTTTTATGTTATTTTAACTTAAAG
CATCTCGGAGCAAAATAATACTACAAGAAATATAGAAACACACTTTGAATCTCTAGAAAT
AGACGACTAAACAAGTTTTGTTGGAATTTTCTTTTTATCTTTTGCATCATTAAAGCACAA
AACTACGTAAACAA

Last Updated on 06.12.2018 16:11