Sequences from confirmation amplicon


Confirmation sequences for line 057A04 and hit At5g52600 are shown.
Line ID 057A04
Locus At5g52600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21343807
Primers used for PCR TC03 - 8474, show primer details
   
Sequence Name 07-K020068-022-057-A04C-TC03
GenBank Accession LN489060 [GenBank]
Sequencing primer gene specific primer, TC03: 5'-TTGTGAGCCAAACAAAAGTTACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K020068-022-057-A04C-TC03
TAAAAAGATGGTTTCTTGTTGTAATAATTATCTTTTGGTACTATAATGTTTTTCGAAAGT
TATTGACCTATATGGAATTTTCAGATTTTAATCGTGGTGTATCTTATGATATCATATTAA
AAAAGAAAATAAGTTGAATCGTGTTTTCTTTTTAAATGTAAGTTACCAAATTCCATAATA
TTAAAATCTGAAAACTAAGAACTAGCTAGGACGTAGATGCATATATCACTCAAATATGTA
TATTAGACATATACCTCTCTTTTCGTTCTTAGATTTTTTAGATATTGATTTGTTATCCCT
TATACACTAGTCTTGACTATTGGAAAAGCATTTTGAGCGAGAAAGAATATTTGAAACGCA
ACAACTGTAAACGCACAAACTGTATCTGCAATGCAATGGACTAACGCATGTATCGAGGTT
GATGGCAATCTTCAACCTCTGATCTCCCGTGCTTATATGTTTGCCTTAAAAGCCAATAAT
TTGTGACAATGTTGAGGATAAAACAAGGAAAGAGCTTTTCAGCTCTAGTTTGCACGAATT
TACAGTAGTGTTACTGTCGGAAAAGTGCAAACCACTGTTCTCTTTTTCTCTTCAATAATT
TTAGTAGATAGTCCCCACATGTAAGAGTTAAAGTTGGAAACCTCAAACTTTT
   
Sequence Name 08-K020068-022-057-A04C-8409
GenBank Accession LN489526 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K020068-022-057-A04C-8409
TAGATTTCCCGGACATGAAGCCATGTAGTAAACACAAAAATTTGGAAATCGAACCGGATC
TACGAGAAATGTCTGCCCAGTTTCCTTCACCATGAGTCTCAACATAGCTTTTTAATATCA
TATCTTCTTCTGGTTTCCACAACCCTCTCTTCACGTAAGACTTCCCTTCTTCTCTTTTGC
ATTCCATCTTTTATTTTTTCTTCCACAGTTTTGCAAGTGAGTGAGGCTGTTTATTAACTA
TATACACAAAAAGTTTGAGGTTTCCAACTTTAACTCTTACATGTGGGGACTATCTACTAA
AATTATTGAAGAGAAAAAGAGAACAGTGGTTTGCACTTTTCCGACAGTAACACTACTGTA
AATTCGTGCAAACTAGAGCTGTAAAAGCTCTATTCCCTTGTTTTTATCCTCAACATTGTC
ACAAATTATTGGCTTTTAAGGCAAACATATAAGCACGGGAGATCAGAGGTTGAAGATTGC
CATCAACCTCGATACTGCGTTAGTCATTGCATTGCAGATACAGTTTGTGCGTTTACAGTT
GTTGCGTTTCAAATATTCTTTCTCGCTCAAAATGCTTTTCCAATAGTCAAGACTAGTGTA
TAAGGGATAACAAATCAATATCTAAAAAATCTAAGAACGAAAAGAGAGGTATATGTCTAA
TATACATATTTGAGTGATATATGCATCT

Last Updated on 06.12.2018 16:11