Sequences from confirmation amplicon


Confirmation sequences for line 058A08 and hit At5g51930 are shown.
Line ID 058A08
Locus At5g51930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21103324
Primers used for PCR AY16 - 8474, show primer details
   
Sequence Name 41-K018335-022-058-A08C-AY16
GenBank Accession LN503905 [GenBank]
Sequencing primer gene specific primer, AY16: 5'-ACGCATTATGCAGACAAGTCATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K018335-022-058-A08C-AY16
ACATCTAGGAAAATCCTGACCCAATCCATTGCAGTCCTTTTAAAATCTTTTGATGTTAAA
TTGGAAGTGAGGATGAATGGGGGTTTGATCTTTCAAAAAGTCGATGGACCGGCCTCAAAA
GGACATATGAAGCTACGGAACACGAACCCAAGGGATAATCCTTCAGTGACATTCAACTAC
TATCAAGAACCAGAGGATTTGAACAAGTGTGTTAAAGGGCTTAACACCATTATTAGAATG
ATAAATTCCAAAGCTTTCTCCAAGTACAAGTATCCGGGTGTGACGGCACGCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACATTC
   
Sequence Name 42-K018335-022-058-A08C-8409
GenBank Accession LN504295 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K018335-022-058-A08C-8409
CGTCACACCCGGATACTTGTACTTGGAGAAAGCTTTGGAATTTATCATTCTAATAATGGT
GTTAAGCCCTTTAACACACTTGTTCAAATCCTCTGGTTCTTGATAGTAGTTGAATGTCAC
TGAAGGATTATCCCTTGGGTTCGTGTTCCGTAGCTTCATATGTCCTTTTGAGGCCGGTCC
ATCGACTTTTTGAAAGATCAAACCCCCATTCATCCTCACTTCCAATTTAACATCAAAAGA
TTTTAAAAGGACTGCAATGGATTGGGTCAGGATTTTCCTAGATGTAGTATGACTTGTCTG
CATAATGCG
Primers used for PCR FM36 - 8474, show primer details
   
Sequence Name 21-K029299-022-058-A08C-8409
GenBank Accession LN495538 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K029299-022-058-A08C-8409
ATTGAATATCCGGATATCATGTTGGCCCTTCCCATCAATTTGAGGCCAAGGCACGTGACC
TCAGCGTTCAACTTGAAGCAGTTTTGCATCGACACCGTGACGAGTGTTTGGCATTACCAT
GGAGGTTGCCAAGTTGGAAAAGTGGTTGACAAGAATTACAAAGTCTTAGGCATAGATGGC
TTAAGGGTCATCGATGGATCCACATTTCTCAAGTCTCCCGGGACTAATCCTCAAGCCACG
GTCATGATGCTTGGAAGGTAAGCACAAAAACTAAATAATCACAACATCAATAAATAAGAT
ATGATGTTTGAATAAAAATCATTGTGAAAGTGCTATGTGTAGGTACATGGGGCAGGAAAT
TATTCGGCAAAGAGCGGCTTTCCACGAAAAGTAACAAAAAGCCTAAGGTTCTTAATAGAG
AGTAGACAAAAGTTTTCTCATCTATTATTACTTGTTGTGTTATTGTCTTCTAACATTTTC
ACACAAATGAAGAAAAGTAAACAAATTTTATTTCTACCCGTTACTAATACACTAGATAAT
ACTTTCTCTTTCTAAATGATAAAGTGAAATAATAGAGATAAAAAAAAAAAAAAGAGATTT
GGTTTTAAATTTTCCTTTGAAAACACAAAAATGACATTTTTTGTGAAACAAAATTAAAAC
CTTAA
   
Sequence Name 22-K029299-022-058-A08C-FM36
GenBank Accession LN495983 [GenBank]
Sequencing primer gene specific primer, FM36: 5'-CCTCCATGGTAATGCCATATAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K029299-022-058-A08C-FM36
GCGTGTCATTTTAAGGTTTTAATTTTGTTTCACAAAAATTGTCATTTTTGTGTTTTCAAT
GGAATATTTAAAACCAAATCTCTTTTTTTTTTTTTTACCCCTATTATTCCCCTTTACCAT
TTAAAAAGAAAAGGTATTACCTAGGGTTTTAGAACCGGGAAAAAAAAAAATTGGTTTCCT
TTCCTCCATTGGGGGGAAAAGGTTAAAAAACAATACCCCACCAGGTAATAAAAGAGGAAA
AAACTTTGGCCCCCCCCCTATAAAAAACCTTAGGCTTTTTGTTCCTTTTCGGGGAAAGCC
CCCCTTTGCCAAAAAATTTCCGGCCCCAGGTCCCTCCCCAAAGCATTTTCCCAAGGATTT
TTATCCAAACCCCAAACCTAATTTATGGAGGTGGGGATTATTAAGTTTTGGGGCTAACCT
CCCAAGCACCAGGACCGGGGCTGGGGGATTACCCCCGGGAAACTTGAAAAAGGGGGACCC
CTCGATGACCCTTAACCCATCTATGCCAAAAACTTTGTAATTCTTGTCACCCACTTTTCC
AACTGGGCAACCCCCAGGGAAAGGCCAAACCCCCGCCCCGGGGTCAATCCAAAACTGCTT
CAATTTAAACCCTAAGGTCACGTCCCTTGGCCTCAAATTGAGGGGAAGGGC

Last Updated on 06.12.2018 16:11