Sequences from confirmation amplicon


Confirmation sequences for line 058C10 and hit At4g00295 are shown.
Line ID 058C10
Locus At4g00295 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:127867
Primers used for PCR DM12 - 8474, show primer details
   
Sequence Name 67-K101747-0022-058-C10C-8409
GenBank Accession LN511776 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K101747-0022-058-C10C-8409
GCCATTTTTTCATCGTCGTTGTATTGACAATCCGTCCATGTTACCGGACGTGTGTTGAAT
GCAAGATTAGTGAAATCGCTACGTAAGTGCCAATCATTAAAAGTGCGAGTAGGCACTACC
ATCATCCTTGCTGGCATATACGTACGCGTGATTTGAACTGTGTATCCCCACGAGATTGAC
ATTGTCCACTGATGACTCTTATCATAGCATACCGATTGTTGGACAAGACTCGCTGAATCG
AGTTTCGCTGGGATCATTAGCTTCTTTAATGCGTTGACTCGGTTTGTTTTTGGGAATATA
GGATCCACTACGTCTAGGTGATGGATTGATACTATTGGTGCTTGTGGATGGNACAGATAT
AGCCCT
   
Sequence Name 67-K101753-0022-058-C10C-DM12
GenBank Accession LN511777 [GenBank]
Sequencing primer gene specific primer, DM12: 5'-TAGGGCTATTATCTGTCCATCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K101753-0022-058-C10C-DM12
TAGACGTAGTGGATCCTATATTCCCAAAACAAACCGAGTCAACGCATTAAAGAAGCTAAT
GATCCCAGCGAAACTCGATTCAGCGAGTCTTGTCCAACAATCGGTATGCTATGATAAGAG
TCATCAGTGGACAATGTCAATCTCGTGGGGATACACAGTTCAAATCACGCGTACGTATAT
GCCAGCAAGGATGATGGTAGTGCCTACTCGCACTTTTAATGATTGGCACTTACGTAGCGA
TTTCACTAATCTTGCATTCAACACACGTCCGGTAACATGGACGGATTGTCAATACAACGA
CAATGGAAAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TC

Last Updated on 06.12.2018 16:11