Sequences from confirmation amplicon


Confirmation sequences for line 058D02 and hit At5g25420 are shown.
Line ID 058D02
Locus At5g25420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8838464
Primers used for PCR PD50 - 8474, show primer details
   
Sequence Name 33-K036417-022-058-D02C-8409
GenBank Accession LN500909 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K036417-022-058-D02C-8409
TGAGCCATTTACAATTGAGTAAGCACTATCACTCTTATCACATCTGCCAACTGTTTTGTG
TATTCACATAAAAGGAATTCTCAATTCGATAAGTTTCAAAAAAGTGTTTGCGATTTAAAT
AATGTGTCTCAAAGTACCTAAGAAGATGTACTTACCCAATTTGAGCGATGATCAGACCGC
CAACCTCCATTGTAGTATTCTCTGAAGTATTGTGGGATTGAAATAGCCATGAAAAAAGAA
AATCCCAAAATGAATTTGGTGTTGAAGCTATTGAGGTTGCAAAATTGTAGAAAGCTAAGT
CCCGCAGAAGCTGCAATATATTAAATTTGATTTTGGTTTTAGTAAAAAGTATAATACATA
TATTTATAGGAAATGTAATCTTAACACTTATAGAAATTACAAGTGTGATGTGATGAAGCG
ACTTACACACAAAACACAAGACGATGCAATAAAGAGATGCCATGATTGGTAATGGTATGG
ACGCAAAAAAAGCTCCAAATTTTCCTGCATTTTGACCAAAATAAATAAGTTGCTAAACCA
GTAAAAGATATTCATAACATCATTTATACAATACAAATAGAAAGATATAGGTTAAACAAA
AAAAATATACCAAAAATGGAGAAAAAAAGCATGAATGCTGCTGATATTTGTA
   
Sequence Name 41-K036417-022-058-D02C-PD50
GenBank Accession LN503994 [GenBank]
Sequencing primer gene specific primer, PD50: 5'-TGATACAAATATCAGCAGCATTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K036417-022-058-D02C-PD50
TGGTATATTTTTTTTGTTTAACCTATATCTTTCTATTTGTATTGTATAAATGATGTTATG
AATATCTTTTACTGGTTTAGCAACTTATTTATTTTGGTCAAAATGCAGGAAAATTTGGAG
CTTTTTTTGCGTCCATACCATTACCAATCATGGCATCTCTTTATTGCATCGTCTTGTGTT
TTGTGTGTAAGTCGCTTCATCACATCACACTTGTAATTTCTATAAGTGTTAAGATTACAT
TTCCTATAAATATATGTATTATACTTTTTACTAAAACCAAAATCAAATTTAATATATTGC
AGCTTCTGCGGGACTTAGCTTTCTACAATTTTGCAACCTCAATAGCTTCAACACCAAATT
CATTTTGGGATTTTCTTTTTTCATGGCTATTTCAATCCCACAATACTTCAAAGAATACTA
CAATGGAGGTTGGCGGTCTGATCATCGCTCAAATTGGGTAAGTACATCTTCTTAGGTACT
TTGAGACACATTATTTAAATCGCAAACACTTTTTTGAAACTTATCGAATTGAGAATTCCT
TTTATGTGAATACACAAAACAGTTGGCAGATGTGATAAGAGTGATAGTGCTTACTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTA
TAGGCGAAAGCAAATAAACAAATTTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11