Sequences from confirmation amplicon


Confirmation sequences for line 059H06 and hit At4g21200 are shown.
Line ID 059H06
Locus At4g21200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11304898
Primers used for PCR MR99 - 8474, show primer details
   
Sequence Name 33-K017447-022-059-H06C-MR99
GenBank Accession LN500793 [GenBank]
Sequencing primer gene specific primer, MR99: 5'-ACCCTTTTTCCAATTTTGAGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K017447-022-059-H06C-MR99
CTATATTATTTCTCTTTTTGGATACTCTTTATATGCATGCATCTATGTATATAGACTATA
GTTCATTTGACATTTGAATGATGAACTCTATTCACTTTATCATCCGGTTGGATTAGCTCG
GTCTAGAAAGTATTGGACATTTGGAAGACTATACCACAATCCGGTCAGTCTAATATATAA
ATACCAGACGGTAATAAAGGGATTATACATGTTTTGTTTTGTTTTGTTTTTTTCAAAGAG
ATTAAGGATCAAAAGTTATTAAAAAAAATTAATACAAAAAAAATTCATCTCTAATTTTAT
TTTTTAAAAATAAAGTATTGCATCTTATTTTCAACTGAAATTTTAGCAAAATGATCGGTG
ATGCATGTGTTTTAGCCTTTTTAGGCATTTGCATTCAAGTGTTTTCTAAACCATTTTTGT
CTACAATATATTAGATCACTGTAAAACCATAATAAAATTCGGATCTATATTTCTATTATC
TCATGGATTATAGTTTGAACTTTCCACATACATTTTCTTATATGTGTATATATATATATA
TTGAATTGGGGTGTTATAATTAATAACATTTTAGATTTTATTGTCAAATTGCCACACTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
   
Sequence Name 34-K017447-022-059-H06C-8409
GenBank Accession LN501218 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K017447-022-059-H06C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTAGTGTGGCAATTTGACAATAAAATCTAAA
ATGTTATTAATTATAACACCCCAATTCAATATATATATATATACACATATAAGAAAATGT
ATGTGGAAAGTTCAAACTATAATCCATGAGATAATAGAAATATAGATCCGAATTTTATTA
TGGTTTTACAGTGATCTAATATATTGTAGACAAAAATGGTTTAGAAAACACTTGAATGCA
AATGCCTAAAAAGGCTAAAACACATGCATCACCGATCATTTTGCTAAAATTTCAGTTGAA
AATAAGATGCAATACTTTATTTTTAAAAAATAAAATTAGAGATGAATTTTTTTTGTATTA
ATTTTTTTTAATAACTTTTGATCCTTAATCTCTTTGAAAAAAACAAAACAAAACAAAACA
TGTATAATCCCTTTATTACCGTCTGGTATTTATATATTAGACTGACCGGATTGTGGTATA
GTCTTCCAAATGTCCAATACTTTCTAGACCGAGCTAATCCAACCGGATGATAAAGTGAAT
AGAGTTCATCATTCAAATGTCAAATGAACTATAGTCTATATACATAGATGCATGCATATA
AAGAGTATCCAAAAAGAGAAATAATATAGAAGACTCA
Primers used for PCR FM38 - 3144, show primer details
   
Sequence Name 57-K029301-022-059-H06C-3144
GenBank Accession LN509309 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K029301-022-059-H06C-3144
ATCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGA
CAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGA
GAACGCCAACTATGAAAAGTAACAACGGTATGTGGTCACACATACACAAACACGCACAGA
AGCTGAAACAATGCATAAAGATGTGTAAGACAACCACATGCATGCAAATACATATGTACA
TATAAGAATGTGTACAGATTCCAGCACACATTAGTACACACTGATCACAAGATAATTTAT
ATACGTGTGTATTTATATATAGCTAGCTAGCCTGGCATGTCCGAGTACTTCTTCTTCCCT
TCCATTATCCCTCATTTGCCAAACAGACAGTTTTCTCGTGACTCTTCCTTTTCTTTTC
   
Sequence Name 58-K029301-022-059-H06C-FM38
GenBank Accession LN509599 [GenBank]
Sequencing primer gene specific primer, FM38: 5'-GAGAAAAGAAAGGAAGAGTCACGA-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K029301-022-059-H06C-FM38
ATGGAAGGGAAGAAGAAGTACTCGGACATGCCAGGCTAGCTAGCTATATATAAATACACA
CGTATATAAATTATCTTGTGATCAGTGTGTACTAATGTGTGCTGGAATCTGTACACATTC
TTATATGTACATATGTATTTGCATGCATGTGGTTGTCTTACACATCTTTATGCATTGTTT
CAGCTTCTGTGCGTGTTTGTGTATGTGTGACCACATACCGTTGTTACTTTTCATAGTTGG
CGTTCTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTA
CCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATA
GTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGA
AGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGT
Primers used for PCR 41wn - 3144, show primer details
   
Sequence Name 44-K118466-0022-059-H06C-41wn
GenBank Accession LN505340 [GenBank]
Sequencing primer gene specific primer, 41wn: 5'-GAAAATGGGATTTAGACCAACTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K118466-0022-059-H06C-41wn
AAACATTAACCACTCATGGTTCCCGAAAAAAAAAACCCATCAACCAACTCATTAGAAGAC
ATAAGTTGCTTTTCAAAACAATATATAAACATTATACTACGAGTCTGCGAACGTTTATAT
CTGAAAACTTTCAAACAATTAACACGCCTATACATAACTGGAATCAAACTAACCATTTTA
CATTTACAATATCTTAGTTATATCTCTTTTTTTTTGGCCCAAAAAAAATTATGTATCAAA
ACTTTTTTCATAGGGGGGTCCAATAAAAAATTTTGGAATACCCTTTCCCAAAAAAAAAAA
AAAAATTTTTCCCAAAAAAATTTTTATTTTACCCAAAACCTCTAAAAACAGGCTGGGTCC
AATCTAAAAACTTTTGGAACCCCCTTTTGGGGTATAAAACAAACCTCCGGTCCTTTTGGG
GGAAAAAAAAAGGGAAAATTCCCAAAAAACCTGTTTGTTGGGCAAAGGAGGAATAAGGGA
GGGAAAAAAAAATTCCTCGAACTTGCCGGGTTACCTGGTTTTATATAAATCCCCCCGTAT
ATAATTTATCTGGGGACCAGGGGGAACAAAGGGGGGCGGAAATCGGAACACTTTCTAATA
TGAACATATGTTTTTGCAGGCAGGGGGTTGTCTAACACATCTTTAGGCATGGTTCCACCT
TCGGGGCGGGTTTGTGTATGTGGAACCACATCCGGTGGTTACTTTTCAAAGTGGGCGTTC
TCA
   
Sequence Name 17-K118465-0022-059-H06C-35St
GenBank Accession LN494064 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118465-0022-059-H06C-35St
ACCTTTCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAG
ATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAGAACGCCAACTATGAAAAG
TAACAACGGTATGTGGTCACACATACACAAACACGCACAGAAGCTGAAACAATGCATAAA
GATGTGTAAGACAACCACATGCATGCAAATACATATGTACATATAAGAATGTGTACAGAT
TCCAGCACACATTAGTACACACTGATCACAAGATAATTTATATACGTGTGTATTTATATA
TAGCTAGCTAGCCTGGCATGTCCGAGTACTTCTTCTTCCCTTCCATTATCCCTCATTTGC
CAAACAGACAGTTTTCTCGTGACTCTTCCTTTCTTTTCTCACCATATAGTACTGTAGTTA
TGTATTATACACAAATAGTGTGTACTATATGTTTCTAGATCGTACCTAGCTTGTTTTTAG
AGGTTCTGAGTAAACTAAAATTTTATTTGAGAAAATTCTTTTCTTTTTTTTTGGTAAAGG
TTATTCAAAAATTCTTAATTGAACACACATATGAAAAAAGTTATGATACATAATCTCTTT
TGGACAAAAAAAAAAGAGATTTACCTAAAATTTTGTAAATGTAAAAGGGTTAGTTTGATT
CCAGTTATGTATAGGCGTGTTAATTGTTTGAAAGTTTTCAAATATAAACTTTCGCAAACT
CGAAGTATAATGTTAATATATGGTTTTGAAAAGCAACTAAGGTCTCCTAATGAGTTGGTT
GATGGGTTTTTTTTTTCGAACCAAGAATTGGGTAAAGGGTTTTCAATAATTT

Last Updated on 06.12.2018 16:11