Sequences from confirmation amplicon


Confirmation sequences for line 062A09 and hit At4g03364 are shown.
Line ID 062A09
Locus At4g03364 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1476681
Primers used for PCR 02wt - 8474, show primer details
   
Sequence Name 49-K118610-0022-062-A09C-02wt
GenBank Accession LN506993 [GenBank]
Sequencing primer gene specific primer, 02wt: 5'-AAATCATCAACGCTCATCAAGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K118610-0022-062-A09C-02wt
AGATGATCACGATTCACGAACATTCAAGATTTGTCTGAGAAGGAGAGGATTCCGAAGAAG
CCGATTGTCGTATGCGTTTTGCCGTATTCCGGTGAGAGTGAAGCTGCCAAGGGGATTCCG
GTGGCTGTGAATGTTAAGATGAACGATAACGTGAAAGAACTAAGAAACGAGTTGGTGAAG
ATTGCAGAGAATGGTGAGTTAAATCTGCCTCAAGAACAGAGTATTTAACGAGAATCAGTC
ATTCTGGGTGGTTGGTGTTGATCACAACGATACAATTGAGATCATACCTAGACATTTTAC
TCCTGGCTCTTTTAACGTTGACTCTTAAGTCTCATTTCTTTGAGTAGCAAAAAAATTGTA
CATTTCCATACATCGAACAAAAGCTGTAAATACAACTCTTAAGTCTCTTGCTTTGTTCAA
AGTTTGCTTTCAGTGCCTGGGAAATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATTA
   
Sequence Name 13-K118609-0022-062-A09C-8409
GenBank Accession LN492263 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K118609-0022-062-A09C-8409
GCCATTTACATTGATTTCCCAGGCACTGAAAGCAAACTTTGAACAAAGCAAGAGACTTAA
GAGTTGTATTTACAGCTTTTGTTCGATGTATGGAAATGTACAATTTTTTTGCTACTCAAA
GAAATGAGACTTAAGAGTCAACGTTAAAAGAGCCAGGAGTAAAATGTCTAGGTATGATCT
CAATTGTATCGTTGTGATCAACACCAACCACCCAGAATGACTGATTCTCGTTAAATACTC
TGTTCTTGAGGCAGATTTAACTCACCATTCTCTGCAATCTTCACCAACTCGTTTCTTAGT
TCTTTCACGTTATCGTTCATCTTAACATTCACAGCCACCGGAATCCCCTTGGCAGCTTCA
CTCTCACCGGAATACGGCAAAACGCATACGACAATCGGCTTCTTCGGAATCCTCTCCTTC
TCAGACAAATCTTGAATGTTCGTGAATCGTGATCATCTCTTGGACTGAGTTTGACGATGG
AGATTGCTCTGTTTGAGCCGTCACAGTGGAATCTTGATGAGCGTGAATGATT

Last Updated on 06.12.2018 16:11