Sequences from confirmation amplicon


Confirmation sequences for line 062C10 and hit At4g26640 are shown.
Line ID 062C10
Locus At4g26640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13437611
Primers used for PCR IS39 - 8474, show primer details
   
Sequence Name 10-K018975-022-062-C10C-IS39
GenBank Accession LN490443 [GenBank]
Sequencing primer gene specific primer, IS39: 5'-GCTTGATTTGAATCTGTGATTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K018975-022-062-C10C-IS39
GATTAAAACTTTATTTTAACTTTATGTAGGAGGATGGAGGGTGCGATGGAAATAACTCCA
CTAGTGAAACCCATCCGGGAGCCTCGGGTTGTTGTTCAAACTCTGAGTGAGGTTGACATT
CTGGATGATGGTTATAGATGGCGCAAATATGGGCAGAAAGTCGTAAGGGGGAACCCAAAT
CCCAGGTTTGTACAGCATTAAAGCTTTTAACACCCCAAAAAATTCCTTGCTTTTTTCTCG
AATGAACATACATAAACATAACTTGCGAGTTCAAAATGGTAGCTAGAACAGTTTAGTCCT
TGTCTTCAGGACCTAATTATTTGACATGTGCATAAAGTGTTGCTTCTTTTAGGAATTTGG
TTTGGAATATGGAAGATAGTGATCTTAACAGACTCAACATGTGCGCAATGGAGATATTAT
GTTTCATGATGTTCCTCGGCCTTTTGTAGGAGCTACTACAAATGCACAGCTCATGGATGC
CCAGTGAGAAAAC
   
Sequence Name 14-K018975-022-062-C10C-8409
GenBank Accession LN492301 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K018975-022-062-C10C-8409
CATGTAGATTTCGTGATTGCTGCTAGACTTTGAAGTGGGAACATCATGATCGTGTTTGCC
TTCGTATGTTGTTATTACAGCTTTTGGATCATGTGATGCTCTCTCCACGTGTTTTCTCAC
TGGGCATCCATGAGCTGTGCATTTGTAGTAGCTCCTACAAAAGGCCGAGGAACATCATGA
AACATAATATCTCCATTGCGCACATGTTGAGTCTGTTAAGATCACTATCTTCCATATTCC
AAACCAAATTCCTAAAAGAAGCAACACTTTATGCACATGTCAAATAACTAGGTCCTGAAG
ACAAGGACTAAACTGTTCTAGCTACCATTTTGAACTCGCAAGTTATGTTTATGTATGTTC
ATTCGAGAAAAAAGCAAGGAATTTTTTGGGGTGTTAAAAGCTTTAATGCTGTACAAACCT
GGGATTTGGGTTCCCCCTTACGACTTTCTGCCCATATTTGCGCCATCTATAACCATCATC
CAGAATGTCAACCTCACTCAGAGTTTGAACAACAACCCGAGGCTCCCGGATGGGTTTCAC
TAGTGGAGTTATTTCCATCGCACCCTCCATCCTCCTACATAAAGTTAAAATAAAGTTTTA
ATCATGTCAAATCACA
Primers used for PCR FM45 - 3144, show primer details
   
Sequence Name 07-K029297-022-062-C10C-3144
GenBank Accession LN489138 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K029297-022-062-C10C-3144
CTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGCAGAGGACAGGG
TACCCGGGGATCAGACCAGATGAAACAGACACCATCAGCCTCAATCTTGGTGTTGGAATC
TCATCTGATGGACCTAACCACGCTTCCAACGAACATCAGCACCAGAATCAACAACTTGTC
AACCAAACTCACCCAAATGGAGTCAATTTCAGGTTTGTTCATGCTAGTCCCATGTCATCC
TACTATGCTAGCTTAAATAGCGGTATGAATCAGTACGGCCAGAGAGAAACAAAGAACGAG
ACTCAAAATGGTGACATCTCGTCCTTGAACAATTCATCTTACCCATATCCGCCCAACATG
GGGAGAGTACAATCGGGTCCGTAAAACAAAAAGTAAGCAACATTATGTACGGGATCTTCT
TAGGTNAAGGAATG
   
Sequence Name 08-K029297-022-062-C10C-FM45
GenBank Accession LN489603 [GenBank]
Sequencing primer gene specific primer, FM45: 5'-CCATTCCTAACCTAAGAAGATCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K029297-022-062-C10C-FM45
GGACCGATTGTCTCTCCCCATGTTGGGCGGATATGGGTAAGATGAATTGTTCAAGGACGA
GATGTCACCATTTTGAGTCTCGTTCTTTGTTTCTCTCTGGCCGTACTGATTCATACCGCT
ATTTAAGCTAGCATAGTAGGATGACATGGGACTAGCATGAACAAACCTGAAATTGACTCC
ATTTGGGTGAGTTTGGTTGACAAGTTGTTGATTCTGGTGCTGATGTTCGTTGGAAGCGTG
GTTAGGTCCATCAGATGAGATTCCAACACCAAGATTGAGGCTGATGGTGTCTGTTTCATC
TGGTCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGA
AGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACA
TCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGT
GGGTGG

Last Updated on 06.12.2018 16:11