Sequences from confirmation amplicon


Confirmation sequences for line 063B01 and hit At3g62860 are shown.
Line ID 063B01
Locus At3g62860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23241721
Primers used for PCR KC41 - 8474, show primer details
   
Sequence Name 62-K035534-022-063-B01C-KC41
GenBank Accession LN510690 [GenBank]
Sequencing primer gene specific primer, KC41: 5'-TATCTATCTCTCTCCCTCTGCTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K035534-022-063-B01C-KC41
AGCAGCATGATGAAGTTCAAGTTCCAGTACGAGGAGGTCTCTGTCTCTCTTGATTTCCAT
CTCTCTCTCTCTCTGCCCATCCTTTAGTTTAGTTGCCTTTTCTTCTTCTTCTATGTTCCT
AATTCCTATGATATATATAGATCAGGCCTGTTCTTAACATTGTAAAACACAGGAATACAT
CAAAAACTCGAGAGACGTTGAGTTGTTCGCTTGCAGATGGCTTCCTTCTTCTTCTCCCCG
AGCTCTAGTCTTCCTCTGCCATGGTAACACCAACTATACTAATAGAAGTTTTATTAATCC
AATTTTATTTGCTTTTTTCTGATTTACTTGATTCACAAAACAAAAAAATCTCCTAATAAT
AATAAGAATTGGTCAATGTGCAGGTTATGGAATGGAGTGCAGTAGCTTCATGAGAGGTAT
TTAATGCTCTCATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAG
ATCTTTATTTCGACGTGTCTACTTTCACGTCCCAATGGG
   
Sequence Name 14-K035534-022-063-B01C-8409
GenBank Accession LN492415 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K035534-022-063-B01C-8409
GAGCCATTTACAATTGAATATATGAGAGCATTAAATACCTCTCATGAAGCTACTGCACTC
CATTCCATAACCTGCACATTGACCAATTCTTATTATTATTAGGAGATTTTTTTGTTTTGT
GAATCAAGTAAATCAGAAAAAAGCAAATAAAATTGGATTAATAAAACTTCTATTAGTATA
GTTGGTGTTACCATGGCAGAGGAAGACTAGAGCTCGGGGAGAAGAAGAAGGAAGCCATCT
GCAAGCGAACAACTCAACGTCTCTCGAGTTTTTGATGTATTCCTGTGTTTTACAATGTTA
AGAACAGGCCTGATCTATATATATCATAGGAATTAGGAACATAGAAGAAGAAGAAAAGGC
AACTAAACTAAAGGATGGGCAGAGAGAGAGAGAGATGGAAATCAAGAGAGACAGAGACCT
CCTCGTACTGGAACTTGAACTTCATCATGCTGCTTCTGATGTCTGATTAAGTGATTATTC
TCGCCGAGCAGAGGGAGAGAGATAGATA

Last Updated on 06.12.2018 16:11