Sequences from confirmation amplicon


Confirmation sequences for line 065B12 and hit At3g09050 are shown.
Line ID 065B12
Locus At3g09050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2764637
Primers used for PCR KM36 - 8474, show primer details
   
Sequence Name 74-K032613-022-065-B12C-KM36
GenBank Accession LN513000 [GenBank]
Sequencing primer gene specific primer, KM36: 5'-GATTTGGAGAGACAGAGAAATTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K032613-022-065-B12C-KM36
TGAAAGTTTGGTGTTAAGGTTGTCTGAAATGCATGAAGTGTGTTCATCATCTTCTATGTT
TTCTTCAACGAAGAAGAAGAAGGCTCGTTTCAGAGTAACCCACCTCTCGTTTTTTAATTT
AGTTTACGCATTATCCAAAACCAGTTTTGAGTTTTAAAGTATTTGCATAAAAGACCCTAT
ATTTGAGACATTATTATAGATTGGCTCTAATATTTCTTAATTACATTAAAGTCATACTTC
CACACATAAATTTCTTTATAAATTGGCCATATTAGCAATTAGCCATTTACATGGACATGA
CCCTATATTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACAT
   
Sequence Name 74-K032613-022-065-B12C-8409
GenBank Accession LN512999 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K032613-022-065-B12C-8409
TACATTGAAATATAGGGTCATGTCCATGTAAATGGCTAATTGCTAATATGGCCAATTTAT
AAAGAAATTTATGTGTGGAAGTATGACTTTAATGTAATTAAGAAATATTAGAGCCAATCT
ATAATAATGTCTCAAATATAGGGTCTTTTATGCAAATACTTTAAAACTCAAAACTGGTTT
TGGATAATGCGTAAACTAAATTAAAAAACGAGAGGTGGGTTACTCTGAAACGAGCCTTCT
TCTTCTTCGTTGAAGAAAACATAGAAGATGATGAACACACTTCATGCATTTCAGACAACC
TTAACACCAAACTTTCATGTTCTTTTCAATGCCTCGAGACATTCTCTTTCCCGACCCCAA
TTTCTCTG

Last Updated on 06.12.2018 16:11