Sequences from confirmation amplicon


Confirmation sequences for line 065E02 and hit At1g03490 are shown.
Line ID 065E02
Locus At1g03490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:872280
Primers used for PCR KM37 - 8474, show primer details
   
Sequence Name 75-K032613-022-065-E02C-8409
GenBank Accession LN513171 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K032613-022-065-E02C-8409
GAAGCCATTACAATTGAAATATCCTGATACTCATTGCACAATCCCATTTTTCTCCAAAAG
GTTGTCTTGTGTCGGATCCGATACAAGAAAGAGGCGAACGTCAACGAGTTTGGACTCGTT
AACCATCAGGCTCATCAGACTCAGGTGGCGGAGAACACCAACAACATCTTACGGAACCAA
GTAGTGTAAGAAGAGGATGCTTTAACCGGTTTTGCTGATCAGCTTGAGATGATGCTAGAG
GGACAAGAAGACCGTGAGCAGAAAGAAGAGGCTGATTTAACCGGTTTTGCTGATAGCCTT
GAGACGATGCTAGAGGGACAAGAAGATCATGAGCAGCCAGAAGATGCTGATTTAACCGGT
TTTGCTGATAGCCTTGAGACGATGCTAGAGGGACATGAAGATCGTGAGCAGCCAGAAGAG
GCTGAATTAACCGGTTTTGCTAATGATG
   
Sequence Name 75-K032613-022-065-E02C-KM37
GenBank Accession LN513172 [GenBank]
Sequencing primer gene specific primer, KM37: 5'-TCATCGTCTCAAGGTCATCATTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K032613-022-065-E02C-KM37
TCTTCATGTCCCTCTAGCATCGTCTCAGGGCTATCAGCCAAACCGGTTAAATCAGCATCT
TCTGGCTGCTCATGATCTTCTTGTCCCTCTAGCATCGTCTCAAGGCTATCAGCAAAACCG
GTTAAATCAGCCTCTTCTTTCTGCTCACGGTCTTCTTGTCCCTCTAGCATCATCTCAAGC
TGATCAGCAAAACCGGTTAAAGCATCCTCTTCTTACACTACTTGGTTCCGTAAGATGTTG
TTGGTGTTCTCCGCCACCTGAGTCTGATGAGCCTGATGGTTAACGAGTCCAAACTCGTTG
ACGTTCGCCTCTTTCTTGTATCGGATCCAACACAAGACAACCTTTTGGAAAAAAATGGGA
TTGTGCAATGAGTATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCATTTT
TTTTTCAATTCAAAAATGTAAATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGAAAATCTTTATTTCGACGTGTCTACATTCA
Primers used for PCR FP09 - 3144, show primer details
   
Sequence Name 37-K032925-022-065-E02C-3144
GenBank Accession LN502515 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K032925-022-065-E02C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTAAG
GGACTGATTAATACTCCATCTTCTGTCAATCGGTCTTGACAAGCTTTCCATGCACTCTCC
TGTGGTACGTGAAAGACTGCTCGTAACCCATCACCCTTTTGCATGTGTCCGTCCTTGATC
TCTTTTTTCCCATCGTTGCTTCTCCAAGTGCCGCCTTCGCTTCCTCCTCTTGCGGCTACC
TTCCGGTGTACGCTTAATATCTTGCCACCTCGTTCAGTAAGCGGAACAAAGTAGTACCAC
TCGTTGTTATTGAACAAAGGATTGTTTACGTGAGGAAGAAGCCAAGGGTAGTCTTGGTGG
ATGTCCATAGTTGTTATGAAACTGCTCTGATTTCTCTCCACCTTGTTTCAAAGATAAA
   
Sequence Name 37-K032925-022-065-E02C-FP09
GenBank Accession LN502516 [GenBank]
Sequencing primer gene specific primer, FP09: 5'-CTTTATCTTCGAAACAAGGTGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K032925-022-065-E02C-FP09
TACACTAGGACATCCCCAGACTACCCTTGGCTTCTTCCTCACGTAAACAATCCTTTGTTC
ATAACAACGAGTGGTACTACTTTGTTCCGCTTACTGAACGAGGTGGCAAGATATTAAGCG
TACACCGGAAGGTAGCCGCAAGAGGAGGAAGCGAAGGCGGCACTTGGAGAAGCAACGATG
GGAAAAAAGAGATCAAGGACGGACACATGCAAAAGGGTGATGGGTTACGAGCAGTCTTTC
ACGTACCACAGGAGAGTGCATGGAAAGCTTGTCAAGACCGATTGACAGAAGATGGAGTAT
TAATCAGTCCCTTAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCC
CGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGA
AGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTG
CTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGG

Last Updated on 06.12.2018 16:11