Sequences from confirmation amplicon


Confirmation sequences for line 068A07 and hit At1g02250 are shown.
Line ID 068A07
Locus At1g02250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:438361
Primers used for PCR CD37 - 8474, show primer details
   
Sequence Name 09-K104193-0022-068-A07C-CD37
GenBank Accession LN490154 [GenBank]
Sequencing primer gene specific primer, CD37: 5'-GCTCTTTTGTTCTTGATCGTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K104193-0022-068-A07C-CD37
ATCTAGTCCTTGTGCATCATTATCAATACCGGTTCCCGGTGTTTTGCTCACCTTAAATCA
CATCATAGAACCCAAAATGCTGTGAGTACTGGCACAAAGTAGCTTGGTTAATTAGCAGTT
TAGAATGGTTGGTCTTTAAGAAAAGGAGATGATAAAGCTCACCTCTTGAGCTAATGACAC
TAACTTGATTGAGTTAGGAGATTCCTGCAAGTCAGTGATTTGGTATAGAGGATTTGAGCT
ACCAAAACTAGGACAAGTGCTTAAAGTTTGAATGGAAATCTGTCGTATTAAACAAAACTA
TAGCTTTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 25-K104194-0022-068-A07C-8409
GenBank Accession LN497419 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K104194-0022-068-A07C-8409
GAGCCTTTACATTGAATATATCCTGCAAGCTATAGTTTTGTTAATACGACAGATTTCCAT
TCAAACTTTAAGCACTTGTCCTAGTTTTGGTAGCTCAAATCCTCTATACCAAATCACTGA
CTTGCAGGAATCTCCTAACTCAATCAAGTTAGTGTCATTAGCTCAAGAGGTGAGCTTTAT
CATCTCCTTTTCTTAAAGACCAACCATTCTAAACTGCTAATTAACCAAGCTACTTTGTGC
CAGTACTCACAGCATTTTGGGTTCTATGATGTGATTTAAGGTGAGCAAAACACCGGGAAC
CGGTATTGATAATGATGCACAAGGGACTGAGATTGGTGAGCATAAATTGGGTCAAGAGAC
GATCAAGACAAAAAGAG

Last Updated on 06.12.2018 16:11