Sequences from confirmation amplicon


Confirmation sequences for line 070F03 and hit At2g31900 are shown.
Line ID 070F03
Locus At2g31900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13562488
Primers used for PCR AS20 - 8474, show primer details
   
Sequence Name 06-K023371w-022-070-F03C-AS20
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, AS20: 5'-TACAAGGCATTACTTCAATGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K023371w-022-070-F03C-AS20
TATCTTGTTATTTGAAGCAGAGAAAACAAACATATTTGATAGAATTGTTCATAAGATTCG
ATCGTCCATTGAGGTAATAATTTAAAGGCAATGACTATGAAAGTAGTATCTGAACCCTCT
GAAACCCGAACTTTATATTCAAGTTATACTAATTTTGGTCCAGAAGGGCCAAGATGACAC
AAGGGAACTAGCTTATTGGCTCACAACAAGCTCTACCCTCTTATATCTTCTCCAATCTAC
ACTCAAGTTCAGCAATACAAATAATGCGGCTTCAAGACGTAACAGATCATCCCATGCTAC
ACTATTTGGGAGATTGGTTCAAGTAAGCTTGGTAAAGTGAACCTCCGATTAATCCATTTT
TTAAGAGCCTCAGTAATAGTTGGTAACAACATTGTAGGGAATGCAACCATCTTCAGTGGG
ATTGGAAACATCCAGCGGTTACAGTGGGATGGCTGGAATACCAAATGACCAAAGCTAAAT
ATCCGGGAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATNTCGACGTGTCTACAT
   
Sequence Name 02-K023371w-022-070-F03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K023371w-022-070-F03C-8409
AGACATGAAGCCATTTACAATTNGAATATTTCCCGGATATTTAGCTTTGGTCATTTGGTA
TTCCAGCCATCCCACTGTAACCGCTGGATGTTTCCAATCCCACTGAAGATGGTTGCATTC
CCTACAATGTTGTTACCAACTATTACTGAGGCTCTTAAAAAATGGATTAATCGGAGGTTC
ACTTTACCAAGCTTACTTGAACCAATCTCCCAAATAGTGTAGCATGGGATGATCTGTTAC
GTCTTGAAGCCGCATTATTTGTATTGCTGAACTTGAGTGTAGATTGGAGAAGATATAAGA
GGGTAGAGCTTGTTGTGAGCCAATAAGCTAGTTCCCTTGTGTCATCTTGGCCCTTCTGGA
CCAAAATTAGTATAACTTGAATATAAAGTTCGGGTTTCAGAGGGTTCAGATACTACTTTC
ATAGTCATTGCCTTTAAATTATTACCTCAATGGACGATCGAATCTTATGAACAATTCTAT
CAAATATGTTTGTTTTCTCTGCTTCAAATAATCTCCATTGAAG

Last Updated on 06.12.2018 16:11