Sequences from confirmation amplicon


Confirmation sequences for line 071E10 and hit At1g34000 are shown.
Line ID 071E10
Locus At1g34000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12358401
Primers used for PCR BF66 - 8474, show primer details
   
Sequence Name 02-K029908-022-071-E10C-8409
GenBank Accession LN486907 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K029908-022-071-E10C-8409
CCATTTACAATTGAATATATCCTGACATGAAAATTGCATCAGCAATTGGACATGGATTGT
ATTTAGTCATGGACATGGTTTCAAGGTAGCTCTGGTATTGAATAAACAAGTTACTAGTCA
TTGATGTTTCTTCAAATTCAGACATATATAGCCTAAAGTTTCACACTTGTCAGTTGGTAC
TGCCTAGACAAGAGATAACTCTGTGTGTTTTGTTTGTAGGTGGGCTATGTTTGGTTTCGC
AGTTGGTATGCTCACAGAGTATGCAACAGGATCAGACCTTGTTGATCAAGTTAAGATCCT
TCTCTCAAATTTCGGCATTCTAGACTTGGAATAATAAGAGTGTTGTTGTTATATATAGAC
AGATCCTTTTCTCTCTTCTCTTCGATTCCTCTTCTTGTTGTTTCTTCTTCTTCTTCCTGT
ATATGTATCTATATAATACAGGGCCATTTGTGTATTCAATCACTTTTATCTTCAAATGGT
ATAAATACGATCTTGTATCATTGATTCTAGAATTGCATACAAAAGATGTTTTGAGGATTA
AATGGAAAGTGTATTCTCGTTTTCAAAAGTATAATAATCTCAGAGCTTTGTTAAGGAATC
TGTGCAATCTCCAGAAGGATTTTGTGGAGAGATTGAGG
   
Sequence Name 02-K029908-022-071-E10C-BF66
GenBank Accession LN486908 [GenBank]
Sequencing primer gene specific primer, BF66: 5'-TTCTTCTCAAAGCCTCAATCTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K029908-022-071-E10C-BF66
GAATGCCAATTCCTTAACAAGCTCTGAAGATTATTATACTTTTGAAAACGAGAATACACC
TTTCCATTTAATCCTCAAAACATCTTTTGTATGCAATTCTACAATCAATGATACAAGATC
GTATTTATACCATTTGAAGATAAAAGTGATTGAATACACAAATGGCCCTGTATTATATAG
ATACATATACAGGAAGAAGAAGAAGAAACAACAAGAAGAGGAATCGAAGAGAAGAGAGAA
AAGGATCTGTCTATATATAACAACAACACTCTTATTATTCCAAGTCTAGAATGCCGAAAT
TTGAGAGAAGGATCTTAACTTGATCAACAAGGTCTGATCCTGTTGCATACTCTGTGAGCA
TACCAACTGCGAAACCAAACATAGCCCACCTACAAACAAAACACACAGAGTTATCTCTTG
TCTAGGCAGTACCAACTGACAAGTGTGAAACTTTAGGCTATATATGTCTGAATTTGAAGA
AACATCAATGACTAGTAACTTGTTTATTCAATACCAGAGCTACCTTGAAACCATGTCCAT
GACTAAATACAATCCATGTCCAATTGCTGATGCAATTTTCATGTCAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTA
TAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11