Sequences from confirmation amplicon


Confirmation sequences for line 072B11 and hit At1g14360 are shown.
Line ID 072B11
Locus At1g14360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4911896
Primers used for PCR RK40 - 8474, show primer details
   
Sequence Name 53-K015908-022-072-B11C-8409
GenBank Accession LN507979 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K015908-022-072-B11C-8409
GTAGATTTCCCGGACATGAAGCCATCAAAGGCAAGGTTTAAGAAGCAAAGCCCGTAACCA
AGGGGTGCATTGGGATGTGCTAGCTTGCTGATGGTTTTAGAGCTTGTCTGTAAACCAAGT
CAAAGACGGTTTACTACATTTCCACAGACAAAGTATAACAAAGATTATGATCAAAGTTCC
TGAATGCAGATTTGCCTACCTTAAGAAGGGCAAACATAGATACTCCTCCGGCAACAAGAA
AGGTGCAAAGATACTCAGGCAAAGTGTATCTTATGCCATAAACTAAGGAGCCCATCAGCA
TAACTACATGAAAGAAAAAACATATATAAGATACACATAGCAGCAGTTGAGAGCGATTAT
GGATTGATGCTCTAAATTGACACCTCACC
   
Sequence Name 54-K015908-022-072-B11C-RK40
GenBank Accession LN508318 [GenBank]
Sequencing primer gene specific primer, RK40: 5'-AGGTGAGGTGTCAATTTAGAGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K015908-022-072-B11C-rk40
AATCCATAATCGCTCTCAACTGCTGCTATGTGTATCTTATATATGTTTTTTCTTTCATGT
AGTTATGCTGATGGGCTCCTTAGTTTATGGCATAAGATACACTTTGCCTGAGTATCTTTG
CACCTTTCTTGTTGCCGGAGGAGTATCTATGTTTGCCCTTCTTAAGGTAGGCAAATCTGC
ATTCAGGAACTTTGATCATAATCTTTGTTATACTTTGTCTGTGGAAATGTAGTAAACCGT
CTTTGACTTGGTTTACAGACAAGCTCTAAAACCATCAGCAAGCTAGCACATCCCAATGCA
CCCCTTGGTTACGGGCTTTGCTTCTTAAACCTTGCCTTTGATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11