Sequences from confirmation amplicon


Confirmation sequences for line 079D09 and hit F6E13 are shown.
Line ID 079D09
Locus F6E13 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18238302
Primers used for PCR YH02 - 8474, show primer details
   
Sequence Name 39-K021056-022-079-D09C-8409
GenBank Accession LN503204 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K021056-022-079-D09C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAAACTATTAATCATTCTTTATTT
ATATAAATAGTTGTATAATTCTGCATCAAGAAAACAAGTTTTACAAATGAAATATTAATC
TCTTACCCTGAAACCTTATGTAATGACAATAGACAAAACCTCCCTTTGTTTAAGGATTTC
TTTCTTCAATTAAAGAAACGTTTATTACATAAAAGTGGAAGAAGAAGAAACATGTTTGGA
ATTAGAAGAAGGCATGAAAGCAAGAACCATAAGCAAAACCATAATCCCAATAGGAATCAA
AAGCAGCATAAAAGGAGGAGGAGGCAATGGTGGAAGAATCAACGGTAAGATCAAGAGAGA
TGCTGTGAGACCAACAAGCACAACCATTGATTCTAAACTGAAGTAACTCGCTGATATTAG
CCTCCTTGGACTGTTCTGCGACGACAATGTCCGAAACAATTCTTGCTTCCCCATAATCGG
AGCTGGAGCTGAACCACGAAAACTCGTGTTTTTCCGGTTATGATCTCCTCTCACGTCCAT
GTTGTTGTTGAGAGAATAATGTTCTTGAATGTTTATGATGTCGTTTTGTA
   
Sequence Name 40-K021056-022-079-D09C-YH02
GenBank Accession LN503560 [GenBank]
Sequencing primer gene specific primer, YH02: 5'-TTTAAATGATTCGTGAGTTCTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K021056-022-079-D09C-YH02
TCTACAAAACGACATCATAAACATTCAAGAACATTATTCTCTCAACAACAACATGGACGT
GAGAGGAGATCATAACCGGAAAAACACGAGTTTTCGTGGTTCAGCTCCAGCTCCGATTAT
GGGGAAGCAAGAATTGTTTCGGACATTGTCGTCGCAGAACAGTCCAAGGAGGCTAATATC
AGCGAGTTACTTCAGTTTAGAATCAATGGTTGTGCTTGTTGGTCTCACAGCATCTCTCTT
GATCTTACCGTTGATTCTTCCACCATTGCCTCCTCCTCCTTTTATGCTGCTTTTGATTCC
TATTGGGATTATGGTTTTGCTTATGGTTCTTGCTTTCATGCCTTCTTCTAATTCCAAACA
TGTTTCTTCTTCTTCCACTTTTATGTAATAAACGTTTCTTTAATTGAAGAAAGAAATCCT
TAAACAAAGGGAGGTTTTGTCTATTGTCATTACATAAGGTTTCAGGGTAAGAGATTAATA
TTTCATTTGTAAAACTTGTTTTCTTGATGCAGAATTATACAACTATTTATATAAATAAAG
AATGATTAATAGTTTCCATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAA

Last Updated on 06.12.2018 16:11