Sequences from confirmation amplicon


Confirmation sequences for line 079F11 and hit At1g15240 are shown.
Line ID 079F11
Locus At1g15240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5243929
Primers used for PCR KM47 - 8474, show primer details
   
Sequence Name 14-K032921-022-079-F11C-8409
GenBank Accession LN492401 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K032921-022-079-F11C-8409
TTACAATTGAATAATCCTGTAATTGAAATCTATATGGATAAGAACAACACCAAACTTACG
GGACATGAGGTAGGAAAGTCCGACGACGCATATGACCACGATTACAATTCGTTTCTTGGC
TTCATCTACCAGGTCCCGAATAGTCACCACTTGCTTCTGGGTGCTCATAGTTGAATCCGT
CTTTTCTGATCTCACACACGCTTGCTTAATCCGTCCTTCAATGAAATTACCAGACAGAGC
GGACGGATTTCTTCCAGAACTTAAGGACCCATTCCCGGGGAATTAGGACCCGATCTTCGA
AAAACGTCCGGATCAGGAAAGATGAGCTGGTTTAGAAACAATGAAAAGGGAAGAGAGGGA
GAGAGAATCAATGGCTGGTANTTCAAATA
   
Sequence Name 14-K032921-022-079-F11C-KM47
GenBank Accession LN492402 [GenBank]
Sequencing primer gene specific primer, KM47: 5'-GTATTTGACTACCCAGCCATTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K032921-022-079-F11C-KM47
CTTTCTTGTTTCTAACAGCTCATCTTTCCTGATCCGGACGTTTTTCGAAGATCGGGTCCT
AATTCCCCGGGAATGGGTCCTTAAGTTCTGGAAGAAATCCGTCCGCTCTGTCTGGTAATT
TCATTGAAGGACGGATTAAGCAAGCGTGTGTGAGATCAGAAAAGACGGATTCAACTATGA
GCACCCAGAAGCAAGTGGTGACTATTCGGGACCTGGTAGATGAAGCCAAGAAACGAATTG
TAATCGTGGTCATATGCGTCGTCGGACTTTCCTACCTCATGTCCCGTAAGTTTGGTGTTG
TTCTTATCCATATAGATTTCAATTACAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11