Sequences from confirmation amplicon


Confirmation sequences for line 079H07 and hit At2g40950 are shown.
Line ID 079H07
Locus At2g40950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17090421
Primers used for PCR Z17a - 8474, show primer details
   
Sequence Name 23-K014703-022-079-H07C-8409
GenBank Accession LN496342 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K014703-022-079-H07C-8409
TGTAGATTTCCCGGACATGAAGCCATTTACATGAACTCATTCCTCCATAACCCCTTTCTT
CCTTTTGAATCTCTCCTTCTTCTTCTTCATCCCCAAATTCGAAACCCTAACCCTAAAACT
CAGTTTCAAAATGGCTGAACCAATCACCAAGGAGCAGCCTCCTCCACCTGCTCCGGACCC
TAATTCCACCTACCCTCCTCCGTCCGATTTTGATTCCATCTCGATCCCTCCGTTAGATGA
TCATTTCTCCGATCAGACTCCGATTGGTGAACTAATGTCCGATCTGGGGTTTCCCGATGG
TGAATTCGAGCTCACTTTCGACGGTATGGACGA
   
Sequence Name 24-K014703-022-079-H07C-Z17a
GenBank Accession LN496781 [GenBank]
Sequencing primer gene specific primer, Z17a: 5'-ATCGTCCATACCGTCGAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K014703-022-079-H07C-Z17a
TGAGCTCGAATTCACCATCGGGAAACCCCAGATCGGACATTAGTTCACCAATCGGAGTCT
GATCGGAGAAATGATCATCTAACGGAGGGATCGAGATGGAATCAAAATCGGACGGAGGAG
GGTAGGTGGAATTAGGGTCCGGAGCAGGTGGAGGAGGCTGCTCCTTGGTGATTGGTTCAG
CCATTTTGAAACTGAGTTTTAGGGTTAGGGTTTCGAATTTGGGGATGAAGAAGAAGAAGG
AGAGATTCAAAAGGAAGAAAGGGGTTATGGAGGAATGAGTTCATGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACG

Last Updated on 06.12.2018 16:11