Sequences from confirmation amplicon


Confirmation sequences for line 080A02 and hit F14G24 are shown.
Line ID 080A02
Locus F14G24 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19713567
Primers used for PCR EV82 - 8474, show primer details
   
Sequence Name 39-K037323-022-080-A02C-EV82
GenBank Accession LN503274 [GenBank]
Sequencing primer gene specific primer, EV82: 5'-TGGAAAGATGATGAATGATCAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K037323-022-080-A02C-EV82
GAATGTATACTTCCTTTGTCTTACTGTTATTTTAGTTTTGTCTTGTTTTCGCTCACTATC
AAACATAAACAAAGTTTTGTCTTGCAATGATATGTTTCTAAGTTTCTTGACATCATTGAA
AGTAAGGAACAAGCAACTCAGGATCATATCTTCACTTTCTCTTCTGAACGCTATTAATCT
TTGCACAAGCATCGCCTTTAAAATTAGTTCTACATATAACTGATTATCAACATCTCTGAT
GCAGCGCATCTCAAATCGTTCTTCAAGTTTGGGCTAGCTCGTTATTTGGGTTTTGTTTCT
CAAGGGCTTAATGTTTATTTGGGCTTTTAGGCTAGAGTTTTCATTATCTTTCTTTTATTT
AAAGAGACGTAATTCATTATTGTATAGTCACAGGCTGATACTTGAATCACTTGCGCAGAG
AATGCACTCCCGCTCAATCGATATACCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACTT
   
Sequence Name 47-K037323-022-080-A02C-8409
GenBank Accession LN506127 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K037323-022-080-A02C-8409
CCATTTCAATTGGTATATCGATTGAGCGGGAGTGCATTCTCTGCGCAAGTGATTCAAGTA
TCAGCCTGTGACTATACAATAATGAATTACGTCTCTTTAAATAAAAGAAAGATAATGAAA
ACTCTAGCCTAAAAGCCCAAATAAACATTAAGCCCTTGAGAAACAAAACCCAAATAACGA
GCTAGCCCAAACTTGAAGAACGATTTGAGATGCGCTGCATCAGAGATGTTGATAATCAGT
TATATGTAGAACTAATTTTAAAGGCGATGCTTGTGCAAAGATTAATAGCGTTCAGAAGAG
AAAGTGAAGATATGATCCTGAGTTGCTTGTTCCTTACTTTCAATGATGTCAAGAAACTTA
GAAACATATCATTGCAAGACAAAACTTTGTTTATGTTTGATAGTGAGCGAAAACAAGACA
AAACTAAAATAACAGTAAGACAAAGTGAAGTGATACAATTTTCCTGAACTCTGATCATTC
TCATTCTTTCC

Last Updated on 06.12.2018 16:11