Sequences from confirmation amplicon


Confirmation sequences for line 080B09 and hit At1g43700 are shown.
Line ID 080B09
Locus At1g43700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16485874
Primers used for PCR MJ04 - 8474, show primer details
   
Sequence Name 35-K017280-022-080-B09C-MJ04
GenBank Accession LN501625 [GenBank]
Sequencing primer gene specific primer, MJ04: 5'-ATGCAATTGGTGTGACTAGTGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K017280-022-080-B09C-MJ04
TAGATTACTAAATATCTTTAAAGCTTTGTGTTTTACGAAGCTGATGTGATTAGTGAAGAT
TACTCTAAGGAACTATTTCTGATATCTTGATTTGAGCTTGGCAGCTTGTAAGCTTGTATT
GTTGCTTAGTGTGTTTAAGATTGCTTCTTGGTGTATAGAGAGGACATGTGTTTGCTACTA
GGAACAAGTATAGATGCAGTGTTATAAGATATGTTATTCTCATGTTAATTTAGCTGATTA
ATCAACATGTGAGATATGTCAATAGTGTAATGTATACACTTGCAAGAGTGTAAGTTTTGT
TTCCACTTTAGTGATAAAAGCAACATAGGAGTAAGATAAAATGATTCATGTCTCTTGCAT
CTCTAATCTGTGCTTTTTCTTATATTGTGTAGAGAGGAACATCAGAGCTGAACACTGAAA
ATAAACACCTCAAAATGCGGCTTCAAGCTTTAGAGCAACAAGCTGAACTTAGGGATGGTA
AAGCCATTTTCTTTTTCATTGGGTCGTTCTCAATATGTAAACCAAAGTCCTCATTACGAT
ATATATTGTTCGGTGTAATTTTCAGCTTTGAATGAAGCGCTGCGGGATGAACTGAACCGA
CTTAAGGTGGTAGCTGGAGAAATTCCTCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTT
   
Sequence Name 36-K017280-022-080-B09C-8409
GenBank Accession LN502032 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K017280-022-080-B09C-8409
ATAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAGGAATTTCTCCAGC
TACCACCTTAAGTCGGTTCAGTTCATCCCGCAGCGCTTCATTCAAAGCTGAAAATTACAC
CGAACAATATATATCGTAATGAGGACTTTGGTTTACATATTGAGAACGACCCAATGAAAA
AGAAAATGGCTTTACCATCCCTAAGTTCAGCTTGTTGCTCTAAAGCTTGAAGCCGCATTT
TGAGGTGTTTATTTTCAGTGTTCAGCTCTGATGTTCCTCTCTACACAATATAAGAAAAAG
CACAGATTAGAGATGCAAGAGACATGAATCATTTTATCTTACTCCTATGTTGCTTTTATC
ACTAAAGTGGAAACAAAACTTACACTCTTGCAAGTGTATACATTACACTATTGACATATC
TCACATGTTGATTAATCAGCTAAATTAACATGAGAATAACATATCTTATAACACTGCATC
TATACTTGTTCCTAGTAGCAAACACATGTCCTCTCTATACACCAAGAAGCAATCTTAAAC
ACACTAAGCAACAATACAAGCTTACAAGCTGCCAAGCTCAAATCAAGATATCAGAAATAG
TTCCTTAGAGTAATCTTCACTAATCACATCAGCTTCGTAAAACACAAAGCTTTAAAGATA
TTTAGTAATCTACTCACCAACCTTCACTAGTCAC
Primers used for PCR 08lf - 8474, show primer details
   
Sequence Name 82-K113838-0022-080-B09C-08lf
GenBank Accession LN514219 [GenBank]
Sequencing primer gene specific primer, 08lf: 5'-AACTATTTCTGATATCTTGATTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K113838-0022-080-B09C-08lf
TGTTTAGATTGCTTCTTGGTGTATAGAGAGGACATGTGTTTGCTACTAGGAACAAGTATA
GATGCAGTGTTATAAGATATGTTATTCTCATGTTAATTTAGCTGATTAATCAACATGTGA
GATATGTCAATAGTGTAATGTATACACTTGCAAGAGTGTAAGTTTTGTTTCCACTTTAGT
GATAAAAGCAACATAGGAGTAAGATAAAATGATTCATGTCTCTTGCATCTCTAATCTGTG
CTTTTTCTTATATTGTGTAGAGAGGAACATCAGAGCTGAACACTGAAAATAAACACCTCA
AAATGCGGCTTCAAGCTTTAGAGCAACAAGCTGAACTTAGGGATGGTAAAGCCATTTTCT
TTTTCATTGGGTCGTTCTCAATATGTAAACCAAAGTCCTCATTACGATATATATTGTTCG
GTGTAATTTTCAGCTTTGAATGAAGCGCTGCGGGATGAACTGAACCGACTTAAGGTGGTA
GCTGGAGAAATTCCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATTC
   
Sequence Name 91-K113839-0022-080-B09C-8409
GenBank Accession LN515132 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K113839-0022-080-B09C-8409
AATATATCCTGAGGAATTTCTCCAGCTACCACCTTAAGTCGGTTCAGTTCATCCCGCAGC
GCTTCATTCAAAGCTGAAAATTACACCGAACAATATATATCGTAATGAGGACTTTGGTTT
ACATATTGAGAACGACCCAATGAAAAAGAAAATGGCTTTACCATCCCTAAGTTCAGCTTG
TTGCTCTAAAGCTTGAAGCCGCATTTTGAGGTGTTTATTTTCAGTGTTCAGCTCTGATGT
TCCTCTCTACACAATATAAGAAAAAGCACAGATTAGAGATGCAAGAGACATGAATCATTT
TATCTTACTCCTATGTTGCTTTTATCACTAAAGTGGAAACAAAACTTACACTCTTGCAAG
TGTATACATTACACTATTGACATATCTCACATGTTGATTAATCAGCTAAATTAACATGAG
AATAACATATCTTATAACACTGCATCTATACTTGTTCCTAGTAGCAAACACATGTCCTCT
CTATACACCAAGAAGCAATCTTAAACACACTAAGCAACAATACAAGCTTACAAGCTGCCA
AGCTCAAATCAAG

Last Updated on 06.12.2018 16:11