Sequences from confirmation amplicon


Confirmation sequences for line 080B09 and hit At1g43710 are shown.
Line ID 080B09
Locus At1g43710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16487236
Primers used for PCR 09lf - 8474, show primer details
   
Sequence Name 83-K113838-0022-080-B09C-09lf
GenBank Accession LN514339 [GenBank]
Sequencing primer gene specific primer, 09lf: 5'-ACAACGTCACTCGCTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K113838-0022-080-B09C-09lf
AGAGCTTATTGGCTAGTGTTCTTGCTCGTTACCGGAAAACTTTGGTTGAACGAACCAAAA
ACCATTTAGGTAAAATTTGATCTGATCTGATCTTTTTTTTTGAATCGGAATCGGAATCTG
AATCGGAATCTGGTGTAGGTTATCCTTATAACTTGGATTTCGACTATGGTGCGCTTGGTC
AGTTACAACATTTTTCGATTAATAATCTTGGAGATCCGTTTATTGAAAGTAACTATGGTG
TACACTCAAGACCTTTTGAAGTTGGTGTGTTGGATTGGTTTGCTCGTCTTTGGGAGATTG
AGAGAGATGATTATTGGGGTTACATTACCAATTGTGGTACTGAAGGCAACCTTCATGGCA
TTTTAGTCGGGTACGTTTTTCGAAAATCATCGGTGTTTTGATTCATTCGAAAAACCGAAA
AAAAACGGTGTTTGATTTGATGTGTGTTTGTAGGAGGGAGATGTTTCCGGATGGGATATT
GTATGCGTCGCGTGAATCGCATTACTCGGTGTTTAAAGCTGCTCGAATGTATCGAATGGA
GTGTGAGAAGGTTGATACGCTTATGTCAGGGGAGATTGATTGTGATGATTTGAGGAAGAA
GTTGTTGGCTAATAAAGATAAACCGGCGATTCTTAATGTTAACATAGGTTGGTTTATGTT
TTAGTACTTTGGCTTTGTTTGTTGTGTTAGGATTGGTGCTAATGGTTGAAATCTTGTTTT
CTTTANGAACGACGGTNAAAGGAGCTGTTGATGATCTTGACCTTGTTATCAAAACTCTNG
AAGAGTGTGGTTTCTCACATGATAGGTTCTATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATG
   
Sequence Name 92-K113839-0022-080-B09C-oriL
GenBank Accession LN515204 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K113839-0022-080-B09C-oriL
CATTTACATTGATAAGAACCTATCATGTGAGAAACACACTCTTCAAGAGTTTTGATAACA
AGGTCAAGATCATCAACAGCTCCTTTAACCGTCGTTCCTAAAGAAAACAAGATTTCAACC
ATTAGCACCAATCCTAACACAACAAACAAAGCCAAAGTACTAAAACATAAACCAACCTAT
GTTAACATTAAGAATCGCCGGTTTATCTTTATTAGCCAACAACTTCTTCCTCAAATCATC
ACAATCAATCTCCCCTGACATAAGCGTATCAACCTTCTCACACTCCATTCGATACATTCG
AGCAGCTTTAAACACCGAGTAATGCGATTCACGCGACGCATACAATATCCCATCCGGAAA
CATCTCCCTCCTACAAACACACATCAAATCAAACACCGTTTTTTTTCGGTTTTTCGAATG
AATCAAAACACCGATGATTTTCGAAAAACGTACCCGACTAAAATGCCATGAAGGTTGCCT
TCAGTACCACAATTGGTAATGTAACCCCAATAATCATCTCTCTCAATCTCCCAAAGACGA
GCAAACCAATCCAACACACCAACTTCAAAAGGTCTTGAGTGTACACCATAGTTACTTTCA
ATAAACGGATCTCCAAGATTATTAATCGAAAAATGTTGTAACTGACCAAGCGCACCATAG
TCGAAATCCAAGTTATAAGGATAACCTACACCAGATTCCGATTCAGATTCCGATTCCGAT
TCAAAAAAAAAGATCAGATCAGATCAAATTTTACCTAAATGGTTTTTGGTTCGTTCAACC
AAAGTTTTCCGGTAACGAGCAAGAACACTAGCCATATAAGCTTCTTTATCTCCGGTGAAT
TCATCGTTAACCTCCGGTTCCGTA

Last Updated on 06.12.2018 16:11