Sequences from confirmation amplicon


Confirmation sequences for line 080D06 and hit At3g07340 are shown.
Line ID 080D06
Locus At3g07340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2342209
Primers used for PCR PH63 - 8474, show primer details
   
Sequence Name 15-K018155-022-080-D06C-PH63
GenBank Accession LN492754 [GenBank]
Sequencing primer gene specific primer, PH63: 5'-AATTGCAAATTTTTGTTGTGATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K018155-022-080-D06C-ph63
CCCAATAACGTTTCTGCTGAAGAATCTAATTGAAGTACTTGTTGATGATGCATTAGATTG
TTGCTTGAAGGAAATATCTGTCAGAAAACAAAAGAAATTAGTTTAAACAAACACACGATT
GGTTCACAAAGACAAAAAAGTAATTATTATTACAATAATTGTCAATTAAAATTACTATAA
ATGTTGTGACGGTGACTTACATCCTTAGACAAGAGAGCGTCCATGTTAAAGTCCAGCCTG
GTGTTCACTGACGATAACTTCATCGACAAGAACTGCACCATATTTGAAATTAAAATTAAG
AAACAAAAATTATTCGAAGTCGTGTAGTTGTTTTAAAAAGTGATTATGAAATAATGGAAA
GAGTTTAAAGTAGACCAATAATCTAATGAGTAATTACCTCAACTTGTCGTTGCAATGATT
GGACATAGTTTATAATTTCATCCAACATCAGTGCTTTTCCAGTAACCTTTAAAAAGTAAA
TTTGTTATTGCAACTATNTTAGTACAACTAANAACGAGTTAATTAGTTGTTTTATAAAAC
TCTCACCTTGTTGCATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAANAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCANAAATG
   
Sequence Name 16-K018155-022-080-D06C-8409
GenBank Accession LN493223 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K018155-022-080-D06C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAATGCAACAAGGTGAG
AGTTTTATAAAACAACTAATTAACTCGTTTTTAGTTGTACTAATATAGTTGCAATAACAA
ATTTACTTTTTAAAGGTTACTGGAAAAGCACTGATGTTGGATGAAATTATAAACTATGTC
CAATCATTGCAACGACAAGTTGAGGTAATTACTCATTAGATTATTGGTCTACTTTAAACT
CTTTCCATTATTTCATAATCACTTTTTAAAACAACTACACGACTTCGAATAATTTTTGTT
TCTTAATTTTAATTTCAAATATGGTGCAGTTCTTGTCGATGAAGTTATCGTCAGTGAACA
CCAGGCTGGACTTTAACATGGACGCTCTCTTGTCTAAGGATGTAAGTCACCGTCACAACA
TTTATAGTAATTTTAATTGACAATTATTGTAATAATAATTACTTTTTTGTCTTTGTGAAC
CAATCGTGTGTTTGTTTAAACTAATTTCTTTTGTTTTCTGACAGATATTTCCTTCAAGCA
ACAATCTAATGCATCATCAACAAGTACTTCAATTAGATTCTTCAGCAGAAACGTTATTGG
GTGATCATCACAACA

Last Updated on 06.12.2018 16:11