Sequences from confirmation amplicon


Confirmation sequences for line 080F09 and hit At4g17490 are shown.
Line ID 080F09
Locus At4g17490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9753926
Primers used for PCR CM15 - 8474, show primer details
   
Sequence Name 13-K027636-022-080-F09C-8409
GenBank Accession LN491887 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K027636-022-080-F09C-8409
GCCATTTACAATTGAATAATCCTGTACACCTTTAATATATCATCCCGCATGTAGTTTGTC
TCTCATTGACTTGACTCTTTCCTATAAATACACCTTTCCACTCTCTCTTCTCCACTGTCA
AATCAAATCCCTCTTCCTCTTCAATTCTCTGTTTCCTCCAAAATGGCTACACCAAACGAA
GTATCAGCTCTTTTCCTCATCAAGAAGTATCTCCTCGACGAATTGTCTCCGTTGCCTACT
ACTGCCACCACCAATCGATGGATGAACGATTTCACGTCATTTGATCAAACCGGTTTCGAG
TTTTCTGAATTTGAAACCAAACCGGAAATAATCGATCTCGTCACTCCCAAACCGGAGATT
TTTGATTTCGATGTGAAATCTGAAA
   
Sequence Name 14-K027636-022-080-F09C-CM15
GenBank Accession LN492358 [GenBank]
Sequencing primer gene specific primer, CM15: 5'-CGATTCAGATGGAATTTCAGATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K027636-022-080-F09C-CM15
TTGGGAGTGACGAGATCGATTATTTCCGGTTTGGTTTCAATTCAGAAAACTCGAAACCGC
GTTTGATCAAATGACGTGAAATCGTTCATCCATCGATTGGTGGTGGCAGTAGTAGGCAAC
GGAGACAATTCGTCGAGGAGATACTTCTTGATGAGGAAAAGAGCTGATACTTCGTTTGGT
GTAGCCATTTTGGAGGAAACAGAGAATTGAAGAGGAAGAGGGATTTGATTTGACAGTGGA
GAAGAGAGAGTGGAAAGGTGTATTTATAGGAAAGAGTCAAGTCAATGAGAGACAAACTAC
ATGCGGGATGATATATTAAAGGTGTACAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATGTCGACGTGTCTACATTCACGTCCAAAT

Last Updated on 06.12.2018 16:11