Sequences from confirmation amplicon


Confirmation sequences for line 081C02 and hit At5g57720 are shown.
Line ID 081C02
Locus At5g57720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23391789
Primers used for PCR KM48 - 8474, show primer details
   
Sequence Name 79-K032613-022-081-C02C-8409
GenBank Accession LN513766 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K032613-022-081-C02C-8409
AGTTTTTCAAGCTCTCTGCAATATATCCGTGTTTATTGCCAATTCCAGTATCAAAGCTCT
CTCTGCAAATGACGTATCTAAAAATTTGCTTTATGACTTTGACTAAACTGATTTGAGTGG
TACAACACACACTCACGCAAAACCCAATCTCACACTCAAAGACTCTCACATACATTAACT
ACATTGTTGAAGATTCTACATAACGCTACAGCTTTTTCATTTGTAGAATCCTGACTCAAG
CAAAGACAAAAAAAGAAGAAGATGGTGTTAGAAAATGGGTTTCCTCCATATCCAGATTTC
CTCAAGATCTTCAACTCCCATGAAGATTCTCAACTTTTGGTAAGTAACTAGTATCTTTCG
TGTTTATCTTCTCCAAATTATCTGTCATGTTAGATTGTAGAATGACTTAAGATTATTTTG
AATTTTTGATGAGATTATTCTTGAATGGTTTGTTGTGTGTTTTGCGTTCGTGTAACCTTA
GTAGATGAATTTCTCTCT
   
Sequence Name 79-K032613-022-081-C02C-KM48
GenBank Accession LN513767 [GenBank]
Sequencing primer gene specific primer, KM48: 5'-CAGAAACAGAGCCAAGAGAGAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K032613-022-081-C02C-KM48
ACGCAAACCACAACAAACCATTCAAGAATAATCTCATCAAAAATTCAAAATAATCTTAAG
TCATTCTACAATCTAACATGACAGATAATTTGGAGAAGATAAACACGAAAGATACTAGTT
ACTTACCAAAAGTTGAGAATCTTCATGGGAGTTGAAGATCTTGAGGAAATCTGGATATGG
AGGAAACCCATTTTCTAACACCATCTTCTTCTTTTTTTGTCTTTGCTTGAGTCAGGATTC
TACAAATGAAAAAGCTGTAGCGTTATGTAGAATCTTCAACAATGTAGTTAATGTATGTGA
GAGTCTTTGAGTGTGAGATTGGGTTTTGCGTGAGTGTGTGTTGTACCACTCAAATCAGTT
TAGTCAAAGTCATAAAGCAAATTTTTAGATACGTCATTTGCAGAGAGAGCTTTGATACTG
GAATTGGCAATAAACACGGATATATTTGCAGAGAGCTTTGAAAAACTTTGTGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACTT
Primers used for PCR FP05 - 8474, show primer details
   
Sequence Name 90-K035343-022-081-C02C-FP05
GenBank Accession LN515005 [GenBank]
Sequencing primer gene specific primer, FP05: 5'-CAGGCCTAATGTTAAAAACTCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K035343-022-081-C02C-FP5
TATACGTGTTACATTGTTTTTCTTTACAACCTTTTTAAAACGTCAAGGTCTCAACCATAT
TTTATTCTCTTGTCCTGATGTCGGCCCATACAGGCCCATATTTGTTTAATGAGTATACAT
GTCTTATGATTGGGCTTATTTGAAACCAGATTTTGAAACAAACCTATCACAAGTGACATG
ACCCATTTTTATATCATTTATTTGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACCATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTG

Last Updated on 06.12.2018 16:11