Sequences from confirmation amplicon


Confirmation sequences for line 083C03 and hit At3g05380 are shown.
Line ID 083C03
Locus At3g05380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1545745
Primers used for PCR TM24 - 8474, show primer details
   
Sequence Name 45-K103693-0022-083-C03C-TM24
GenBank Accession LN505505 [GenBank]
Sequencing primer gene specific primer, TM24: 5'-ACAGCATGCTTTAGATGAAAAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K103693-0022-083-C03C-TM24
TTTTCAATCGGTACATTTGTTCAATGTTTTGGAAATCGAATAGGATCATAATAAATGGAC
CTTTGAGTGCCCACGTAATTCATTCCTTCTGATTAATCTTGAATACACTTATTCTTGTGC
TGTGTGACAAGGAAATGGAGCCAGAGATGCTAGAAATTGTCAAGGGTTCAAAAACAAGAG
CGCAAGCAATGGTGGATGCAGCTATAAAGGTATCGATTCATCCACTTTTCTAGATTCTCG
AACATACATATGTTTTGCTAAGTTATGGTCACTCTACGGTTTCTAGGCTGCATCATCTGT
GAAGGAAGGGGAAGATGTGAACACAATGATCCAAGAAGCCTTAGAGTTGGTTGGCAAAAA
TCAGCTATTACGCAGCTCTATGGTCAAACATCACGAGCATGTAAATGGCAGTATAGAGCA
TCATCACAACCCATCTCCTTCAAATGGATCAGAGCCTGTGGCTAACAACGATTTAAACTC
ACAAGATGGTTCAGAGAAAAACGCTCAAATGCCTTCAGAGCTTATCACCTCCTGTGTTGC
CACTTGGCTCATGATTCAGGTAATTAGGAAATTGCTTAGAAGGATTGCATACCCAACGTG
ATAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCANAAATGT
   
Sequence Name 61-K103694-0022-083-C03C-8409
GenBank Accession LN510499 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K103694-0022-083-C03C-8409
GGTATGCATCCTTCTAGCAATTTCCTAATTACCTGAATCATGAGCCAAGTGGCAACACAG
GAGGTGATAAGCTCTGAAGGCATTTGAGCGTTTTTCTCTGAACCATCTTGTGAGTTTAAA
TCGTTGTTAGCCACAGGCTCTGATCCATTTGAAGGAGATGGGTTGTGATGATGCTCTATA
CTGCCATTTACATGCTCGTGATGTTTGACCATAGAGCTGCGTAATAGCTGATTTTTGCCA
ACCAACTCTAAGGCTTCTTGGATCATTGTGTTCACATCTTCCCCTTCCTTCACAGATGAT
GCAGCCTAGAAACCGTAGAGTGACCATAACTTAGCAAAACATATGTATGTTCGAGAATCT
AGAAAAGTGGATGAATCGATACCTTTATAGCTGCATCCACCATTGCTTGCGCTCTTGTTT
TTGAACCCTTGACAATTTCTAGCATCTCTGGCTCCATTTCCTTGTCACACAGCACAAGAA
TAAGTGTATTCAAGATTAATCAGAAGGAATGAATTACGTGGGCACTCAAAGGTCCATTTA
TTATGATCCTATTCGATTTCCAAAACATTTGAACAAATGTACCGATATGAAAAATAGAAT
ATCAGTAATCCATTACCTTTTCATCTAGC

Last Updated on 06.12.2018 16:11