Sequences from confirmation amplicon


Confirmation sequences for line 085D11 and hit At1g30440 are shown.
Line ID 085D11
Locus At1g30440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10759623
Primers used for PCR PH05 - 3144, show primer details
   
Sequence Name 25-K019985-022-085-D11C-PH05
GenBank Accession LN497243 [GenBank]
Sequencing primer gene specific primer, PH05: 5'-AAAGAGCTCAAAACCAAATTTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K019985-022-085-D11C-PH05
ATTTTTTTTATCCACAAGACTGATAAAACACAATGTATGAAAAGGAAAATAGAAACCAAA
CACAACTTTGATACATTAGCAGTACAGATATTTACAATGCCGAAGGACATAACATTCTAG
AAGACCAATAATTAGTCCAGATAAATGCAATAATATCCCACAGTGTTTCGAGAAGAGAGT
GTTTTAACAAATTGTACCTTGTGGAGATGAAAAGACATCTCCCCAACTTCAACGACAATA
TCACTAGGAAGTCCAGTTGTGCAAAACCTGCAATTAGCAAAAGACCAAAGAAGACAAAGT
CAGACAAAGGAATGAGCTTTGTTTAACTCAGTAATGCTGCTATTTGCCTTAAAAGAAAGA
CCATGGTCTGGATTTGAGCAGACTTTGGTCTGGATTTGAGACCATGGTCTTTCTTTTGTG
CTTCTAATTGAGCACAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATC
CCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGC
GAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACT
TGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGG
   
Sequence Name 26-K019985-022-085-D11C-3144
GenBank Accession LN497719 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K019985-022-085-D11C-3144
GACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGA
AGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTC
GGTTTAAACTATCAGTGTTTGTGCTCAATTAGAAGCACAAAAGAAAGACCATGGTCTCAA
ATCCAGACCAAAGTCTGCTCAAATCCAGACCATGGTCTTTCTTTTAAGGCAAATAGCAGC
ATTACTGAGTTAAACAAAGCTCATTCCTTTGTCTGACTTTGTCTTCTTTGGTCTTTTGCT
AATTGCAGGTTTTGCACAACTGGACTTCCTAGTGATATTGTCGTTGAAGTTGGGGAGATG
TCTTTTCATCTCCACAAGGTACAATTTGTTAAAACACTCTCTTCTCGAAACACTGTGGGA
TATTATTGCATTTATCTGGACTAATTATTGGTCTTCTAGAATGTTATGTCCTTCGGCATT
GTAAATATCTGTACTGCTAATGTATCAAAGTTGTGTTTGGTTTCTATTTTCCTTTTCATA
CATTGTGTTTTATCAGTCTTGTGGATAAAAAAAATCGTTACTGCTGTTCCTTGAAAATTT
GGTT
Primers used for PCR JU09 - 3144, show primer details
   
Sequence Name 37-K019986-022-085-D11C-JU09
GenBank Accession LN502451 [GenBank]
Sequencing primer gene specific primer, JU09: 5'-AAAGAGCTCAAAACCAAATTTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K019986-022-085-D11C-JU09
CAGCAGTAACGATTTTTTTTATCCACAAGACTGATAAAACACAATGTATGAAAAGGAAAA
TAGAAACCAAACACAACTTTGATACATTAGCAGTACAGATATTTACAATGCCGAAGGACA
TAACATTCTAGAAGACCAATAATTAGTCCAGATAAATGCAATAATATCCCACAGTGTTTC
GAGAAGAGAGTGTTTTAACAAATTGTACCTTGTGGAGATGAAAAGACATCTCCCCAACTT
CAACGACAATATCACTAGGAAGTCCAGTTGTGCAAAACCTGCAATTAGCAAAAGACCAAA
GAAGACAAAGTCAGACAAAGGAATGAGCTTTGTTTAACTCAGTAATGCTGCTATTTGCCT
TAAAAGAAAGACCATGGTCTGGATTTGAGCAGACTTTGGTCTGGATTTGAGACCATGGTC
TTTCTTTTGTGCTTCTAATTGAGCACAAACACTGATAGTTTAAACCGAAGGCGGGAAACG
ACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGG
AAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCAC
ATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCG
TGGGTGGG
   
Sequence Name 38-K019986-022-085-D11C-3144
GenBank Accession LN502828 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K019986-022-085-D11C-3144
GACGTAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAA
GTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCG
GTTTAAACTATCAGTGTTTGTGCTCAATTAGAAGCACAAAAGAAAGACCATGGTCTCAAA
TCCAGACCAAAGTCTGCTCAAATCCAGACCATGGTCTTTCTTTTAAGGCAAATAGCAGCA
TTACTGAGTTAAACAAAGCTCATTCCTTTGTCTGACTTTGTCTTCTTTGGTCTTTTGCTA
ATTGCAGGTTTTGCACAACTGGACTTCCTAGTGATATTGTCGTTGAAGTTGGGGAGATGT
CTTTTCATCTCCACAAGGTACAATTTGTTAAAACACTCTCTTCTCGAAACACTGTGGGAT
ATTATTGCATTTATCTGGACTAATTATTGGTCTTCTAGAATGTTATGTCCTTCGGCATTG
TAAATATCTGTACTGCTAATGTATCAAAGTTGTGTTTGGTTTCTATTTTCCTTTTCATAC
ATTGTGTTTTATCAGTCTTGTGGATAAAAAAAATCGTTACTGCTGTTCCTTGAAAATTTG
GT

Last Updated on 06.12.2018 16:11