Sequences from confirmation amplicon


Confirmation sequences for line 086C02 and hit At1g79810 are shown.
Line ID 086C02
Locus At1g79810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30020632
Primers used for PCR 27oz - 8474, show primer details
   
Sequence Name 41-K116218-0022-086-C02C-27oz
GenBank Accession LN504212 [GenBank]
Sequencing primer gene specific primer, 27oz: 5'-AGAGCTAACTTGATGCTTTCCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K116218-0022-086-C02C-27oz
CATTTATAGCCAGGAATGCTCTTTCAATACGAACCAGAGCTTGATGCTTTCCTTGAGTTT
CTCATTTGGAGATTCTCTATTTGGGTTGACAAACCCACCCCCGGAAACGCTCTCATGAAT
CTTAGATACAGAGATGAACGAGGAGTCGTTGCTCAGCACTTGGGGAAAGGTATTTTTTAT
TTTATTTTCTAAACACTCTTCTTCTGTCACCCTTTTTTTCTAATCACTCTTGTACTCTCG
TATAGTCAGAACAGGACTGGAAGGACCCGGACTTACTTCTCCCCAAAAGATTTGGTACTG
CGTTGCCTCCGTTGGTGGTCAGTACCTCTTCTCCCGTTTGCAGTCATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAC
   
Sequence Name 17-K116217-0022-086-C02C-8409
GenBank Accession LN494006 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K116217-0022-086-C02C-8409
ACATTGAATATGACTGCAAACGGGAGAAGAGGTACTGACCACCAACGGAGGCAACGCAGT
ACCAAATCTTTTGGGGAGAAGTAAGTCCGGGTCCTTCCAGTCCTGTTCTGACTATACGAG
AGTACAAGAGTGATTAGAAAAAAAGGGTGACAGAAGAAGAGTGTTTAGAAAATAAAATAA
AAAATACCTTTCCCCAAGTGCTGAGCAACGACTCCTCGTTCATCTCTGTATCTAAGATTC
ATGAGAGCGTTTCCGGGGGTGGGTTTGTCAACCCAAATAGAGAATCTCCAAATGAGAAAC
TCAAGGAAAGCATCAAGCTCTGGTTCGTATTGAAAGAGCATTCCTGGCTAATAAAATGCC
AAAGAAAGTAAATGGCTTAATAAAATGCCAAGGAAAGCATCAGNTTAGCTCTA
Primers used for PCR 13pe - 8474, show primer details
   
Sequence Name 56-K116249-0022-086-C02C-13pe
GenBank Accession LN509207 [GenBank]
Sequencing primer gene specific primer, 13pe: 5'-CATTTTATTAGCCAGGAATGCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K116249-0022-086-C02C-13pe
TGCTTTCTTGAGTTTCTCATTTGGAGATTCTCTATTTGGGTTGACAAACCCACCCCCGGA
AACGCTCTCATGAATCTTAGATACAGAGATGAACGAGGAGTCGTTGCTCAGCACTTGGGG
AAAGGTATTTTTTATTTTATTTTCTAAACACTCTTCTTCTGTCACCCTTTTTTTCTAATC
ACTCTTGTACTCTCGTATAGTCAGAACAGGACTGGAAGGACCCGGACTTACTTCTCCCCA
AAAGATTTGGTACTGCGTTGCCTCCGTTGGTGGTCAGTACCTCTTCTCCCGTTTGCAGTC
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCACGGATTATTATA
   
Sequence Name 32-K116248-0022-086-C02C-8409
GenBank Accession LN500722 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K116248-0022-086-C02C-8409
AATTGAAATGACTGCAAACGGGAGAAGAGGTACTGACCACCAACGGAGGCAACGCAGTAC
CAAATCTTTTGGGGAGAAGTAAGTCCGGGTCCTTCCAGTCCTGTTCTGACTATACGAGAG
TACAAGAGTGATTAGAAAAAAAGGGTGACAGAAGAAGAGTGTTTAGAAAATAAAATAAAA
AATACCTTTCCCCAAGTGCTGAGCAACGACTCCTCGTTCATCTCTGTATCTAAGATTCAT
GAGAGCGTTTCCGGGGGTGGGTTTGTCAACCCAAATAGAGAATCTCCAAATGAGAAACTC
AAGGAAAGCATCAAGCTCTGGTTCGTATTGAAAGAGCATTCCTGGCTAATAAAATG

Last Updated on 06.12.2018 16:11