Sequences from confirmation amplicon


Confirmation sequences for line 087B12 and hit At3g25840 are shown.
Line ID 087B12
Locus At3g25840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9457098
Primers used for PCR 39wt - 8474, show primer details
   
Sequence Name 47-K118610-0022-087-B12C-39wt
GenBank Accession LN506340 [GenBank]
Sequencing primer gene specific primer, 39wt: 5'-AGAAAACGAACTTCTGCCATTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K118610-0022-087-B12C-39wt
TCCGCTTCTTTATCCCACCTCTTATCCTCCCTTTCAGATTCACGAGTTAGAACACCATTA
GAACTCTTTTCTTCTCCTTGACGCCCTTTTTCCCCAAGTGGAGGCTGAAAGCATCGCAAA
AGATATTCAGAATACTTACCAGAAGAGAAATACATGACACTAAAAACGCAAGAACATACA
ATTAAAAAAAAGGGAAAAAGAACATCTACATAAAACCAGTTCTTACAAATCAAGCGAAAG
CCATCAGTCTCCATTGAACGCCTAGTGCAACACTCAGGTTATCTAAAGAAAACTCTCAAA
AGACCAAAGCTTTATCATTAATTCAGGCGTAAATGCTGTTTGTAATATGAAAAAATCAAT
CAAACCCAAATCCACCCCTCTGATCCCACAATCCAAACAGAATTTAGGAAGAAAAAGACG
TATTAGAGGGATTAGAACAGACCAATTTGATGGATCAGCAATGAGTATGATGGATCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
CGGTTATT
   
Sequence Name 11-K118609-0022-087-B12C-oriL
GenBank Accession LN491335 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K118609-0022-087-B12C-oriL
CTATCCTCTATACGTCTTTTTCTTCCTAAATTCTGTTTGGATTGTGGGATCAGAGGGGTG
GATTTGGGTTTGATTGATTTTTTCATATTACAAACAGCATTTACGCCTGAATTAATGATA
AAGCTTTGGTCTTTTGAGAGTTTTCTTTAGATAACCTGAGTGTTGCACTAGGCGTTCAAT
GGAGACTGATGGCTTTCGCTTGATTTGTAAGAACTGGTTTTATGTAGATGTTCTTTTTCC
CTTTTTTTTAATTGTATGTTCTTGCGTTTTTAGTGTCATGTATTTCTCTTCTGGTAAGTA
TTCTGAATATCTTTTGCGATGCTTTCAGCCTCCACTTGGGGAAAAAGGGCGTCAAGGAGA
AGAAAAGAGTTCTAATGGTGTTCTAACTCGTGAATCTGAAAGGGAGGATAAGAGGTGGGA
TAAAGAAGCTGGAGGTCCTTCAGAGAGAGTTAGTAAATTAAGTTATGATAATGGCAGAAG
TTCGTTTTCT
Primers used for PCR 09ws - 8474, show primer details
   
Sequence Name 46-K118610-0022-087-B12C-09ws
GenBank Accession LN506014 [GenBank]
Sequencing primer gene specific primer, 09ws: 5'-ATATGCCACGTTTGCCTTATTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K118610-0022-087-B12C-09ws
GCATTCTGGTCGCGATTCTATACAAAATTTCTTGAGCCTTAACCCTAATTTCGATTACGA
CGATACTCTCTGATGGCGAACGATAAGCAGATCGAATCTCACCACCGCAAGCATCACCGT
TCATCTTCGTCGTCCGATGACACTGAAAAAGCATCGAAACGACACAAGCATCGTCATCAC
AAGCATCATCACCACAGGCATCGCCATCACCGAGATAAGAAGCGCGAAAATGAAATTCCA
TCCGCCGGCGATGAGACAGAGATATTGGATGTTACTCCGGCGGCTCCAATTGTTGTTAGT
AACGGCTGTGAGGAGGAGGATGTGGAGGAAGGAGAGATTTTAGAAGAAGATGGGATTGGT
GATGTTTTGATGAAGACGGCAGATTCCGACGGCGAATCAGGCGAAATCAAATTTGAAGAT
AATAATCTGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
T
   
Sequence Name 10-K118609-0022-087-B12C-8409
GenBank Accession LN490877 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K118609-0022-087-B12C-8409
TATCTTCAATTTGATTTCGCCTGATTCGCCGTCGGAATCTGCCGTCTTCATCAAAACATC
ACCAATCCCATCTTCTTCTAAAATCTCTCCTTCCTCCACATCCTCCTCCTCACAGCCGTT
ACTAACAACAATTGGAGCCGCCGGAGTAACATCCAATATCTCTGTCTCATCGCCGGCGGA
TGGAATTTCATTTTCGCGCTTCTTATCTCGGTGATGGCGATGCCTGTGGTGATGATGCTT
GTGATGACGATGCTTGTGTCGTTTCGATGCTTTTTCAGTGTCATCGGACGACGAAGATGA
ACGGTGATGCTTGCGGTGGTGAGATTCGATCTGCTTATCGTTCGCCATCAGAGAGTATCG
TCGTAATCGAAATTAGGGTTAAGGCTCAAGAAATTTTGGTATAGAATCGCGACCAGAATT
GCTTGTAACATCTTTTTAAAGGGATTTTATGTAAATAAGGCAAACTTGGCATAT

Last Updated on 06.12.2018 16:11