Sequences from confirmation amplicon


Confirmation sequences for line 087C09 and hit At2g36300 are shown.
Line ID 087C09
Locus At2g36300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15213590
Primers used for PCR LB31 - 8474, show primer details
   
Sequence Name 32-K033706-022-087-C09C-8409
GenBank Accession LN500481 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K033706-022-087-C09C-8409
TTGACCAAGCTCGTACACGTATGGAGATTAAGGTTCCATTTAAACCAGCAAGCATGTTAA
AGACGACGTATAGAAAGCTATAGGAGACGACGATCCATCCAAGTATGACACCGAACTGGA
TCTTACCAGCTAACAACTGAAATAAGCAAAGCGCAAGGTACAGAAAGATCGGACCAGATA
GATCTGAATCTTTGTGAACAGCTTGGTTAATCCGAAAAGGATTGAGAATCGATCTAGTCT
TCTTCCAGATTTGATCTGGATGGATACCGAGCTCGTCGAGTAATGGCTCCTCATCTTCAA
ACGAAGCTGAACCTCCACCAAAAGAAGAAGAGGAAGCGATTGTCCCGCCGAATGGTCCGG
CAGGTGTAGCGGAAGACGCGGCTGCGGATCCAATGTCGAATGACATGAAAGGGATTGCGG
AAGAAGAGGGACGCGGCGGTTGAAACGGTGTCGCTGGGAATCTTCGTTGCTGGACATTAG
CACCGCCTGAAGATCCACCGGAGGGGAAAACTACAGGTGGAACTGCGAAATCCTTCGTCA
TCTTCTTTCCGAAATTGAGATCTCACCAAATCAGCAAAGAG
   
Sequence Name 32-K033706-022-087-C09C-LB31
GenBank Accession LN500482 [GenBank]
Sequencing primer gene specific primer, LB31: 5'-ATCTCTTTGCTGATTTGGTGAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K033706-022-087-C09C-LB31
ATTTCGCAGTTCCCCTGTAGTTTTCCCCTCCGGTGGATCTTCAGGCGGTGCTAATGTCCA
GCAACGAAGATTCCCAGCGACACCGTTTCAACCGCCGCGTCCCTCTTCTTCCGCAATCCC
TTTCATGTCATTCGACATTGGATCCGCAGCCGCGTCTTCCGCTACACCTGCCGGACCATT
CGGCGGGACAATCGCTTCCTCTTCTTCTTTTGGTGGAGGTTCAGCTTCGTTTGAAGATGA
GGAGCCATTACTCGACGAGCTCGGTATCCATCCAGATCAAATCTGGAAGAAGACTAGATC
GATTCTCAATCCTTTTCGGATTAACCAAGCTGTTCACAAAGATTCAGATCTATCTGGTCC
GATCTTTCTGTACCTTGCGCTTTGCTTATTTCAGTTGTTAGCTGGTAAGATCCAGTTCGG
TGTCATACTTGGATGGATCGTCGTCTCCTCTATCTTTCTATACGTCGTCTTTAACATGCT
TGCTGGTAGAAATGGGAACCTTAATCTCCATACGTGTACGAGCTTGGTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11