Sequences from confirmation amplicon


Confirmation sequences for line 089C04 and hit At4g34850 are shown.
Line ID 089C04
Locus At4g34850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16609438
Primers used for PCR PH06 - 8474, show primer details
   
Sequence Name 47-K017449-022-089-C04C-PH06
GenBank Accession LN506036 [GenBank]
Sequencing primer gene specific primer, PH06: 5'-CTACTTCAAAACCACCAAGTGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K017449-022-089-C04C-PH06
AACTCAAACAGAAACTTACTCGTCTCTGTAATCTCTTCATTTTAACCTCTGTTTTCAACT
TTTTATCTCATTCTGTAAGCTCCTGCAACAGCAACTGAGGTTTGTTTGAACTGTTCGTTT
ACTATATACAGGCAAGACAACAACGGTGAAGACAAGGTATGTTGTGATGTCAGAGGAGAT
TCTCAAGAAGTACCCAGAACTTGCCATTGAAGGAGGATCCACAGTGACGCAGCGTCTAGA
CATCTGCAACGATGCAGTGACCGAGATGGCAGTAGAAGCCTCCAGAGCCTGCATCAAGAA
CTGGGGACGTTCTATTTCCGACATAACTCACGTTGTCTATGTCTCCTCAAGCGAAGCTCG
TCTTCCTGGTGGGGACTTGTACCTGGCCAAGGGGCTTGGACTCAGTCCTGACACACACCG
TGTTCTGCTCTACTTTGTTGGCTGTTCTGGTGGCGTTGCAGGTCTCCGTGTAGCCAAAGA
CATCGCCGAGAATAATCCGGGCAGTAGAGTTTTGCTTGCCACGTCTGAGACAACCATCAT
TGGGTTCAAGCCCCCAAGTGTGGATAGACCGTATGATCTTGTTGGGGTCGCGTTGTTTGG
TGATGGAGCCGGAGCTATGATCATTGGATCCGATCCAGACCCGATCTGCGAGAAGCCTCT
CTTTGAGCTTCATACTGCAATACAGAACTTTTTACCTGGTAA
   
Sequence Name 48-K017449-022-089-C04C-8409
GenBank Accession LN506365 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K017449-022-089-C04C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATAGTTCTTATGAGCCAGTCC
CGCTTTTCCTATTAGCTTCTTGCAGAAATTCTCCACATTGTCTTCTATTATCTGTGGAAG
CTCTCTCGATAGCTTGAAGTTTATCCCTTGTTCTGTTAGCCTTCCATCTATCGTCTTCTC
TGTTTCTGCGCCATGAAAATGAAAATCCATATTTATCCAAGAATAGTCTCAAGGTATAGA
CATAACTGGATGCATAAATCTTGTGGTCTTTTACCAGGTAAAAAGTTCTGTATTGCAGTA
TGAAGCTCAAAGAGAGGCTTCTCGCAGATCGGGTCTGGATCGGATCCAATGATCATAGCT
CCGGCTCCATCACCAAACAACGCGACCCCAACAAGATCATACGGTCTATCCACACTTGGG
GGCTTGAACCCAATGATGGTTGTCTCAGACGTGGCAAGCAAAACTCTACTGCCCGGATTA
TTCTCGGCGATGTCTTTGGCTACACGGAGACCTGCAACGCCACCAGAACAGCCAACAAAG
TAGAGCAGAACACGGTGTGTGTCAGGACTGAGTCCAAGCCCCTTGGCCAGGTACAAGTCC
CCACCAGG

Last Updated on 06.12.2018 16:11