Sequences from confirmation amplicon


Confirmation sequences for line 089F05 and hit At5g03150 are shown.
Line ID 089F05
Locus At5g03150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:747653
Primers used for PCR TM12 - 8474, show primer details
   
Sequence Name 35-K023014-022-089-F05C-8409
GenBank Accession LN501665 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K023014-022-089-F05C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATACTCAATACTCATTT
CTGTTTTATTGCTCATCACACATAGAGCGTTCTGCGACGCATTAACTGAGGAAGGAGCGA
GGATGAGTTCACTTAGTAACAACAATCCGGTGATCTCTACGACGAATCTGAATTTTGGAA
ATGAGTCAAATGTTATGAATAATCCAAATCTTCCTCATGGATTTGTACACCGAGGAGTTC
ATCATCCCGACATTAATGCTGCTATCTCTCAATTCGGTCTAGGGTTTGGACATGACCTAA
GTGCAATGCATGCGCAAGGTACATCACATACCAATCCTCTTTCTAAATTAGTTATTCATA
TATATGAAATATTTTTTTTTTTTGATGAATATGTTATAATACTATTATCAATTCAATTGC
GTTAATTTAGG
   
Sequence Name 36-K023014-022-089-F05C-TM12
GenBank Accession LN502062 [GenBank]
Sequencing primer gene specific primer, TM12: 5'-TCTCGGATAGACCTAAATTAACGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K023014-022-089-F05C-TM12
CAATTGAATTGATAATAGTATTATAACATATTCATCAAAAAAAAAAAATATTTCATATAT
ATGAATAACTAATTTAAAAAGAGGATTGGTATGTGATGTACCTTGCGCATGCATTGCACT
TAGGTCATGTCCAAACCCTAGACCGAATTGAGAGATAGCACCATTAATGTCGGGATGATG
AACTCCTCGGTGTACAAATCCATGAGGAAGATTTGGATTATTCATAACATTTGACTCATT
TCCAAAATTCAAATTCGTCGTAAAGATCACCGGATTGTTGTTACTAAGTGAACTCATCCT
CGCTCCTTCCTCAGTTAATGCGTCGCAAAACGCTCTATGTGTGATGAGCAATAAAACAGA
AATGAGTATTGAGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATG

Last Updated on 06.12.2018 16:11