Sequences from confirmation amplicon


Confirmation sequences for line 092B09 and hit At1g48220 are shown.
Line ID 092B09
Locus At1g48220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17803366
Primers used for PCR 45ym - 8474, show primer details
   
Sequence Name 11-K120546-0022-092-B09C-45ym
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 45ym: 5'-ATGTTTGAAATCCGTATCCCTATG-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K120546-0022-092-B09C-45ym
GGACATAATGTCTCAGTTGTTCGTGTTTGACAATTTAATTGCTTGAGTAATAAAGAGAGA
TAAGATCATTGATCTTACCATTTTACTCTCTTCTCTTTGTCTTAGATAGGCAAATCTTGT
TTTCAAAACTGTTTGTTGTCTATATTATCTTACACTTTCATAAGTAGGAGATAGAAGCTT
AAGGTGCTATCAAACCCTTTTTTTATACCTTTTGTCACATTTTGTCTTCTGTACTATTAC
AGTTAAGTTAGCTCGTGTGAGAAAAGGGTGTAATATAATGAGCTGTTTTGGCTGGTGTGG
GAGTGAGGATGTTCGTAATCCTGCTGACACCGGACCAAGTCAAGCACATAACTCAATAGG
TAGCTCACATATTTCAACTGCACCACTTGTCTTTGTTTTTCTTATATTGTCCAGTTTTTG
GCTTCTAAGTAAGTTAATAGGAATTGAATCGTTGTAAAGGTTACAATGGACGCCACCATC
AAAGAGCTGATCCACCAATGAACCAACCAGTAGTTAACATGCAGCCTATCGCTGTACCAG
CCATTCCAGTGGATGAACTGGAAGATATAACCGAGAACTTTAGTTCAGAGGTATTAGTCG
GTAAAGGTTCATATGGAAGAGTGTTTTACGGTGTTCTTAAAAGCGGTAAAGAAGCTGCCA
TCAAGAAACTTTACCCTACTAAGCAGCCAGATCAAGAATTTCTTTCCCAGGTAGATACTA
AAATTAAAGTACATCANTTCAGTTTTTACTAACCTTAGCTTAGACCGTATATATGGATCA
TGGCTTCATGTAAATGGCTTCATGTCCCGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCCATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
   
Sequence Name 01-K120545-0022-092-B09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K120545-0022-092-B09C-8409
ATGAAGCCATGATCCATATATACGGTCTAAGCTAAGGTTAGTAAAAACTGAAATGATGTA
CTTTAATTTTAGTATCTACCTGGGAAAGAAATTCTTGATCTGGCTGCTTAGTAGGGTAAA
GTTTCTTGATGGCAGCTTCTTTACCGCTTTTAAGAACACCGTAAAACACTCTTCCATATG
AACCTTTACCGACTAATACCTCTGAACTAAAGTTCTCGGTTATATCTTCCAGTTCATCCA
CTGGAATGGCTGGTACAGCGATAGGCTGCATGTTAACTACTGGTTGGTTCATTGGTGGAT
CAGCTCTTTGATGGTGGCGTCCATTGTAACCTTTACAACGATTCAATTCCTATTAACTTA
CTTAGAAGCCAAAAACTGGACAATATAAGAAAAACAAAGACAAGTGGTGCAGTTGAAATA
TGTGAGCTACCTATTGAGTTATGTGCTTGACTTGGTCCGGTGTCAGCAGGATTACGAACA
TCCTCACTCCCACACCAGCCAAAACAGCTCATTATATTACACCCTTTTCTCACACGAGCT
AACTTAACTGTAATAGTACAGAAGACAAAATGTGACAAAAGGTATAAAAAAAGGGTTTGA
TAGCACCTTAAGCTTCTATCTCCTACTTATGAAAGTGTAAGATAATATAGACAACAAACA
GTTTTGAAAACAAGATTTGCCTATCTNAGACAAAGAGAAGAGAGTAAAATGGTAAGATCA
ATGATCTTATCTCTCTNTATTACTCAAGCAATTAAATTGTCAAACACGAACAACTGAGAC
ATTATGTTCCCTAAACCTAGAATTTTCATAAGTCAACGAGATATAACATAGGG

Last Updated on 06.12.2018 16:11