Sequences from confirmation amplicon


Confirmation sequences for line 094H05 and hit At1g54370 are shown.
Line ID 094H05
Locus At1g54370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20294695
Primers used for PCR RK21 - 8474, show primer details
   
Sequence Name 31-K015896-022-094-H05C-8409
GenBank Accession LN499939 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K015896-022-094-H05C-8409
ATTTCCCGGACCTGAAGCCATTTACAATTGAAACATCAACCCATGTAGCAGAAACAATTG
AATTGCTTCTGAGAGATTTGAGAAAGTGTAGCGCTTCATAACCTACAGTAGCACTATATT
AGGCAGCCTAAATATGTCTTTCCAAGATATTAACATCAACTAAGATGTAAAAGCTACAAT
GACTCGGCGATTACAATTCCTGTGAAGAGTATAGAAACAATGCCGGAGAGACCAACACCT
TCTGCAAGCATGTATCTTACAGAATAACCAGAATACATTAGGGGGAAGAAGTGTTGTCAT
AAATACGAAGAGACAGGAGGAGAGTTGTTTTGTCTACTTACGAAAAATACGGGAAAAGTA
CAAAGAGACAACACTCCAAGTTCTGAAGACTGAGGTAGAGAAAAGAGAAAACAGAGACAT
TACATTTACAATATGTTCAGAGAGTATTAACAATGTAAATACAACAGTATAGAAAGAGTT
AAGAAAAGATATGGCACTTACTTCTCGGTGTCCAATCCTGCATACTTAAAGAGGTGTTCT
GAGTTAAGATTTTACAATACATTTTTGGCCATGTGACATCTAATAAGAAAAATAGAGGAT
TCGAGGAAGGATATTAAAGCTGAAGTGAATCCAACCCCAACCCCTGCAGAATAATATTAA
GACTGTTAGTATGTTTAAGCAATATATGAAGTCGATGAAGGAATC
   
Sequence Name 32-K015896-022-094-H05C-RK21
GenBank Accession LN500363 [GenBank]
Sequencing primer gene specific primer, RK21: 5'-CGGTCGTATTAGTTACCCTTTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K015896-022-094-H05C-rk21
ATCTTTTTCTTCTAAAGTCCTTCACAGTCACATATACATCTACATATATAAGACCTGATT
TTTTTCTATATATATAAGACCTGTTATTTTCTTCGTTTTCTTTTCTCCCAGAATAAATAG
TTCTGCCAATAGCTTCTAACCGATTCCTTCATCGACTTCATATATTGCTTAAACATACTA
ACAGTCTTAATATTATTCTGCAGGGGTTGGGGTTGGATTCACTTCAGCTTTAATATCCTT
CCTCGAATCCTCTATTTTTCTTATTAGATGTCACATGGCCAAAAATGTATTGTAAAATCT
TAACTCAGAACACCTCTTTAAGTATGCAGGATTGGACACCGAGAAGTAAGTGCCATATCT
TTTCTTAACTCTTTCTATACTGTTGTATTTACATTGTTAATACTCTCTGAACATATTGTA
AATGTAATGTCTCTGTTTTCTCTTTTCTCTACCTCAGTCTTCAGAACTTGGAGTGTTGTC
TCTTTGTACTTTTCCCGTATTTTTCGTAAGTAGACAAAACAACTCTCCTCCTGTCTCTTC
GTATTTATGACAACACTTCTTCCCCCTAATGTATTCTGGTTATTCTGTAAGATACATGCT
TGCAGAAGGTGTTGGTCTCTCCGGCATTGTTTCTATACTCTTCACAGGAATTGTAATCGC
CGAGTCATTGTAGCTTTTACATCTTAGTTGATGTTAATATCTTGGAAAGACATATTTAGG
CTGCCTAATA

Last Updated on 06.12.2018 16:11