Sequences from confirmation amplicon


Confirmation sequences for line 095C11 and hit At1g66560 are shown.
Line ID 095C11
Locus At1g66560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24834339
Primers used for PCR IS11 - 8474, show primer details
   
Sequence Name 09-K015960-022-095-C11C-8409
GenBank Accession LN489958 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K015960-022-095-C11C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTACACTGGAGGGTTATC
TTGGATCATCTGCACCCGCTTTCTAGCATTGCAGTTTTGGTCTTTTGCATAGGTACACCG
ATAGTAACACCTGTGAATATAGCACTTTTATTTACATGTATTTCAAAGAATAAAGAAGAG
AATTTACTTTCTTTTTGTAAGAACAGAGAACTTGCTCTGTTTTTCTAGGTAACCTAATTG
ACTAACCTTTGGTACGGTGAGGTTTTGATAGTTTTTTGTCCATATTTCCTCCAGGTGAAG
CCGTCATCGGGGCGTGGAGTCGGGGATTCGTCTCTGTAATACTCTAAATCTTCTGCTCCA
TAATATTTTCTACAATGATGTATTGGAGAAATGCTTAAAAATATAGAAGACATAATTGCA
TATCAAAAATTATACGAGACATAAAAGTAACATGAAAAAACAATTGCTATTTAACTTTAT
TTTGAGTTAGCTAGAAACTCATGAACTTTGTTCGTAACATATGCAATGAGTGTTTAGCTT
TTTGTTGGTAACTTACTTCTTTGATGATCTCTGAAGAACATGACTTGTCATGTTTTGAAG
AG
   
Sequence Name 10-K015960-022-095-C11C-IS11
GenBank Accession LN490423 [GenBank]
Sequencing primer gene specific primer, IS11: 5'-CCACACAATGAGTCTTCTCTTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K015960-022-095-C11C-is11
AAGTCATGTTCTTCAGAGATCATCAAAGAAGTAAGTTACCAACAAAAAGCTAAACACTCA
TTGCATATGTTACGAACAAAGTTCATGAGTTTCTAGCTAACTCAAAATAAAGTTAAATAG
CAATTGTTTTTTCATGTTACTTTTATGTCTCGTATAATTTTTGATATGCAATTATGTCTT
CTATATTTTTAAGCATTTCTCCAATACATCATTGTAGAAAATATTATGGAGCAGAAGATT
TAGAGTATTACAGAGACGAATCCCCGACTCCACGCCCCGATGACGGCTTCACCTGGAGGA
AATATGGACAAAAAACTATCAAAACCTCACCGTACCAAAGGTTAGTCAATTAGGTTACCT
AGAAAAACAGAGCAAGTTCTCTGTTCTTACAAAAAGAAAGTAAATTCTCTTCTTTATTCT
TTGAAATACATGTAAATAAAAGTGCTATATTCACAGGTGTTACTATCGGTGTACCTATGC
AAAAGACCAAAACTGCAATGCTAGAAAGCGGGTGCAGATGATCCAAGATAACCCTCCAGT
GTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11