Sequences from confirmation amplicon


Confirmation sequences for line 098G12 and hit At3g20910 are shown.
Line ID 098G12
Locus At3g20910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7327798
Primers used for PCR BG02 - 8474, show primer details
   
Sequence Name 41-K022408-022-098-G12C-8409
GenBank Accession LN503925 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K022408-022-098-G12C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATATGGAACAAACCCC
TGATAGGTTAAATGTGTTATTTATTATAAAGTCACATAGATGCTAAATAGATAAGAAGAG
CCTAAAAATTCCTGTCGTGAACTAAATACATCTTCTCAATATCTTCAATAAACTGCTGCT
ATAAATCATAATTCATCTATCTATCAGGAACACTGTTAGACAATTATAATCATGCTAGAC
TTCACAAAGCACCTACAGCTAATAGAATATTTAAACATGCAATCTCATGTTTCGCAACCC
AATAGCAATCATGAATCATGGTAGACATGTCACATGGTTAAATCGTCTTAACAGAGATAA
TCAGTTGAGGCTTTAGGAAAACTTGTAAATAGTTTGGAAATAGTCATATACCAGGGGATG
ATGTCCATAGGCTCCCATCACTCCTGCATAATATGGATCCTGGTATGGATTTGAGGATGC
CCATCCCTGCATGATTCATAACCGGGAAAAAACTAAATTGTAAGCAACCATAGC
   
Sequence Name 42-K022408-022-098-G12C-BG02
GenBank Accession LN504314 [GenBank]
Sequencing primer gene specific primer, BG02: 5'-TTGGGAAAATTTTAGCTATGGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K022408-022-098-G12C-BG02
CAATTTAGTTTTTTCCCGGTTATGAATCATGCAGGGATGGGCATCCTCAAATCCATACCA
GGATCCATATTATGCAGGAGTGATGGGAGCCTATGGACATCATCCCCTGGTATATGACTA
TTTCCAAACTATTTACAAGTTTTCCTAAAGCCTCAACTGATTATCTCTGTTAAGACGATT
TAACCATGTGACATGTCTACCATGATTCATGATTGCTATTGGGTTGCGAAACATGAGATT
GCATGTTTAAATATTCTATTAGCTGTAGGTGCTTTGTGAAGTCTAGCATGATTATAATTG
TCTAACAGTGTTCCTGATAGATAGATGAATTATGATTTATAGCAGCAGTTTATTGAAGAT
ATTGAGAAGATGTATTTAGTTCACGACAGGAATTTTTAGGCTCTTCTTATCTATTTAGCA
TCTATGTGACTTTATAATAAATAACACATTTAACCTATCAGGGGTTTGTTCCATATCAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAG

Last Updated on 06.12.2018 16:11