Sequences from confirmation amplicon


Confirmation sequences for line 099A09 and hit F16P2 are shown.
Line ID 099A09
Locus F16P2 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12632838
Primers used for PCR NK15 - 8474, show primer details
   
Sequence Name 10-K102588-0022-099-A09C-8409
GenBank Accession LN490602 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K102588-0022-099-A09C-8409
ACATGAATTGGCCAAAAAATAGACTCTTCAAACAAAAAAAGTTGTGTGTTTTACAAATAC
AGTCGATACATTAATCTCTCAGTTTTAAACTTTTCAACATATGAATCTTAAAATATAAAT
TGTAGAACGTAATCATTTGGAGATTTGTTATTTCTTGGTCGGCTAATTGACTGGAGATTA
GAAACACTAAGTGTTGGTCAAATATCTTAAAACACTAAGACGAATGACGTTGGCAATGAC
GTGCATACCTTAAAATATAAATCAGTCCTTTTCTGGTGTGATCTTCAAGATATCCTTGGA
TACAAAGACAAAGAGAAAGCAAAATGGTCCTTTTATTTATTTGTTTATTTCACCACCCAT
AACTTCATTTGTGTATAGAATATAGTAAACTCAAGTAATCAAGCAAGTAAACGAAACATA
AAAAAGAAAAGTAGTGCAAGAAACAGAGGAGTTAGATACCAAAAAGTGAGAGATATCGAA
TGGCGGAGAAAGAAGAGAGTGTGAAGCTTCTAGGGTTTTGGGCAAGCCCTTTTAGTCGTC
GAGTCGAGATGGCTCTCAAACTCAAAGGCGTGCCATACGAATACTTGGAAGAAGATTTGC
CCAACAAGACCCCTTTGCTTCTGGAGCTAAACCCGCTTCACAAGAAAGTTCCGGTTCTTG
TCCACAATGACAAAATATTACTCGAGTCACATCTGATTCTCGAATACATCGATCAGACGT
GGAAGAACAGTCCAATTCTT
   
Sequence Name 10-K102589-0022-099-A09C-NK15
GenBank Accession LN490603 [GenBank]
Sequencing primer gene specific primer, NK15: 5'-CGTAAGGATCTTGAGGAAGAATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K102589-0022-099-A09C-NK15
ATTCGAGAACAGATGTGACTCGAGTAATATTTTGTCATTGTGGACCCATAAGGAACTTTC
TTGTGAAGCGGGTTTAGCTCCAGAAGCTAAGGGGTCTTGTTGGGCAAATCTTCTTCCAAG
TATTCGTATGGCACGCCTTTGAGTTTGAGAGCCATCTCGACTCGACGACTAAAAGGGCTT
GCCCAAAACCCTAGAAGCTTCACACTCTCTTCTTTCTCCGCCATTCGATATCTCTCACTT
TTTGGTATCTAACTCCTCTGTTTCTTGCACTACTTTTCTTTTTTATGTTTCGTTTACTTG
CTTGATTACTTGAGTTTACTATATTCTATACACAAATGAAGTTATGGGTGGTGAAATAAA
CAAATAAATAAAAGGACCATTTTGCTTTCTCTTTGTCTTTGTATCCAAGGATATCTTGAA
GATCACACCAGAAAAGGACTGATTTATATTTTAAGGTATGCACGTCATTGCCAACGTCAT
TCGTCTTAGTGTTTTAAGATATTTGACCAACACTTAGTGTTTCTAATCTCCAGTCAATTA
GCCGACCAAGAAATAACAAATCTCCAAATGATTACGTTCTACAATTTATATTTTAAGATT
CATATGTTGAAAAGTTTAAAACTGAGAGATTAATGTATCGACTGTATTTGTAAAACACAC
AACTTTTTTTGTTTTGAAGAGTCTATTTTTTGGCCAATTCATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11