Sequences from confirmation amplicon


Confirmation sequences for line 099B01 and hit At1g18880 are shown.
Line ID 099B01
Locus At1g18880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6522169
Primers used for PCR IH42 - 8474, show primer details
   
Sequence Name 88-K100855-0022-099-B01C-8409
GenBank Accession LN514831 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K100855-0022-099-B01C-8409
ATTTACATTGAATATTTCATATCCGGGAAATATTGCCATTTACAAGAGGCGGTTAAAGCC
CGTTGGGCCTAACGAGCTTTACAACTACATCGCCAGTGATTTCAAGAACTCGAAATTGGG
CCATACAGAGCAGTTCAGGTACTAATAACCTCACTACTTCTCTTAGTTTCTTGTGGAACA
ACTTAGAAACATTTTAGGGTTTTCTTAATTTGCAGGTTTCTTGACAAATCGGCAATCCAA
ACACAAGACGACAAGCTGAACAAAGATGGGTCGCCGGTGGATGCATGGAAACTCTGCAGT
ATGCAACAAGTGGAGGAAGTTAAATGCGTGATCCGGGTGCTTCCGGTTTGGCTCTCGGCC
GCCTTATTCTACCTAGCTTACATTCAACAAACAACCTACACAATCTTTCAGTCTCTTCAA
TCCGATAGACGCCTTGGTCCAGGAAGTTTCCAGATCCCGGCCGGTTCTTATACCGTCTTC
CTAATGCTTGGAATGACGATA
   
Sequence Name 88-K100861-0022-099-B01C-IH42
GenBank Accession LN514832 [GenBank]
Sequencing primer gene specific primer, IH42: 5'-AATATCGTCATTCCAAGCATTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K100861-0022-099-B01C-IH42
CGGCCGGGATTGGAACTTCCTGGACCAGGCGTCTATCGGATTGAAGAGACTGAAAGATTG
TGTAGGTTGTTTGTTGAATGTAAGCTAGGTAGAATAAGGCGGCCGAGAGCCAAACCGGAA
GCACCCGGATCACGCATTTAACTTCCTCCACTTGTTGCATACTGCAGAGTTTCCATGCAT
CCACCGGCGACCCATCTTTGTTCAGCTTGTCGTCTTGTGTTTGGATTGCCGATTTGTCAA
GAAACCTGCAAATTAAGAAAACCCTAAAATGTTTCTAAGTTGTTCCACAAGAAACTAAGA
GAAGTAGTGAGGTTATTAGTACCTGAACTGCTCTGTATGGCCCAATTTCGAGTTCTTGAA
ATCACTGGCGATGTAGTTGTAAAGCTCGTTAGGCCCAACGGGCTTTAACCGCCTCTTGTA
AATGGCAATATTTCCCGGATATTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11