Sequences from confirmation amplicon


Confirmation sequences for line 099C03 and hit At1g50370 are shown.
Line ID 099C03
Locus At1g50370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18659169
Primers used for PCR BM01 - 8474, show primer details
   
Sequence Name 07-K015829-022-099-C03C-8409
GenBank Accession LN489034 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K015829-022-099-C03C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATCAAGCAAAATCCTAAAATCCGTA
GACCAAAATGGCAAAGGAAACAGTAAGGGAGAGAGACCAGATTCAAAGTAAAGTGCCACA
AAGCAAGCTCTAAAGTCTCAAAAGAGTAAGCGAAGAATTGGGTTTTTGTAGATGCATGAA
TATTCGGGGAAGAGAAAGCAAGCAAGAGAAGAAAGAAGAAAGAAGAAGAAGGCGTACGTA
TTCGCAGAGAAGTTGAAGCTCGTCCTCAGAGAGATGTTGGCCGTCTTTAACCTTGGAAAT
CCATTGATCTAAATCCATGGCGGCCTTTTATTATCCTCTCTGTCTCTCTCTTTCGGTGGT
GAAGTGGAAATTGGTGCTGTTTCTTCGATTTCAATGGTGAGTCTCGACGATCTCTGGTGA
AGAATTGAAAGAAAGATAGATCTAGCAGAGCATTGCTCCGTCCGCTTAGGGTTACGTAAG
GGGGAAAGAGGGAGAGAGAGAGAAAGGGGAAACACAACGAACCAAACAATCTCGACCCGA
ACCCGATTTGGTCTTCCTCCATCCCCAAACTCTATTCGTTGGATATTTATTTTTAATCAT
TTTTTTGGCAACATATTAAACATCAATTTAATTTTTATCTATTACTTCATTACACAAATC
TTTAATGGAGTATACTACACATTTTCAATGTTTTTGC
   
Sequence Name 08-K015829-022-099-C03C-BM01
GenBank Accession LN489499 [GenBank]
Sequencing primer gene specific primer, BM01: 5'-CCAAGAAAAGAATTGCAAAAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K015829-022-099-C03C-bm01
CTGTGTAGTATACTCCATTAAAGATTTGTGTAATGAAGTAATAGATAAAAATTAAATTGA
TGTTTAATATGTTGCCAAAAAAATGATTAAAAATAAATATCCAACGAATAGAGTTTGGGG
ATGGAGGAAGACCAAATCGGGTTCGGGTCGAGATTGTTTGGTTCGTTGTGTTTCCCCTTT
CTCTCTCTCTCCCTCTTTCCCCCTTACGTAACCCTAAGCGGACGGAGCAATGCTCTGCTA
GATCTATCTTTCTTTCAATTCTTCACCAGAGATCGTCGAGACTCACCATTGAAATCGAAG
AAACAGCACCAATTTCCACTTCACCACCGAAAGAGAGAGACAGAGAGGATAATAAAAGGC
CGCCATGGATTTAGATCAATGGATTTCCAAGGTTAAAGACGGCCAACATCTCTCTGAGGA
CGAGCTTCAACTTCTCTGCGAATACGNACGCCTTCTTCTTCTTTCTTCTTTCTTCTCTTG
CTTGCTTTCTCTTCCCCGAATATTCATGCATTCTACAAAAACCCAATTCTTCGCTTACTC
TTTTGAGACTTTAGAGCTTGCTTTGTGGCACTTTACTTTGAATCTGGTCTCTCTCCCTTA
CTGTTTCCTTTGCCATTTTGGTCTACGGATTTTAGGATTTTGCTTGATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA

Last Updated on 06.12.2018 16:11