Sequences from confirmation amplicon


Confirmation sequences for line 102A03 and hit At1g72450 are shown.
Line ID 102A03
Locus At1g72450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27276218
Primers used for PCR IL15 - 3144, show primer details
   
Sequence Name 23-K032414-022-102-A03C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K032414-022-102-A03C-3144
TATAACTCCACCTTTACCAGCACTTAAAAAATGGTTTTAAACACTTTTACTTCCC
   
Sequence Name 23-K032414-022-102-A03C-IL15
GenBank Accession LN496448 [GenBank]
Sequencing primer gene specific primer, IL15: 5'-AAGCTGTCCAAAATAATACCATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K032414-022-102-A03C-IL15
AAAATGATTTAAAATTTTGCATTTCAACAAAAAAAATATATAGTTAAACTTTATGCTAAT
AATCAACGAAGCAGGATACTAAAAGTATATGATATAAACTTTGGTCTTTATAGGCGGCGG
CCGTCGGAATAGATAGGGAAAAATAAATAAATACCTTTGAGATCGAATTTTCCGGCGAGT
TCAAGATCGGATTTTCGAGCCAACCCCATATTAATATTCCCAAAACTTCCCTTCTCCTTC
AAGTACCGGCTGAGCAGACTACATCTCTGAGAAAAATTGGACTTCTCCGGCGCTTGTCCC
GTTGACATACTAGTGTGATGAAGATTACAACAACCCAAGATTTTCTTGTGTGACTTCTTT
ACACGTACAAAAAATCGAGGATTCCAAAGAACCGAAGGCGGGAAACGACAATCTGATCCC
CGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGA
AGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTG
CTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGT
Primers used for PCR FB09 - 8474, show primer details
   
Sequence Name 89-K034733-022-102-A03C-8409
GenBank Accession LN514917 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K034733-022-102-A03C-8409
GCCATTTACAATTAAAGCTCATGTGTTGTTGTTATAAATAACCAATTCTAGCCAATAAGT
TTCTGTGGCTTTTAACCTCTCCCGAATAAATAAAGAAAACGAAATAACGTGTTCTTTTTT
TTTATTTAGTCTGAAGTGACAGTGATGTGTGTCCAATATAGAATGTTTTTGTCACGAGTT
TTGTAACTTTTTTTTTTACACCTAACTGATCGATAGACATTTTTTTTTTTTTTTTAAACA
AAAAACTGATAAAAAAACAAAACCCCCTTTTTTTTTAAAAGGGTTAAAAAAAAAAAATTT
TTTTTTAGGGCAAAAAATATAAAGGGCAATAATTTTCCAAAACCCCCAAAGGTTTTGCCC
CAAAAAAAAAAAGGAAAAAAAATTAAAGGGGAAAAAAATAATTTTTCCCTAGGGTTAATT
AAAAAAATAAAACAAAAAGGTTCTTTAAAATTTCCGGCCCTTTTTCTTTGCAAAACCCGG
AAAAAAACTGTTGAAATTTAACGGCAACTTTACAAAAGGGGTTTACCCCTTTTAATTCTT
TCGGGCCCCAAAATTTTCTGTAAAAAACAAATTTCCCCTTCAAAGGAAATTCTTTAAGGG
GGGGTAAGAAAATTCTTTA
   
Sequence Name 90-K034733-022-102-A03C-FB09
GenBank Accession LN515004 [GenBank]
Sequencing primer gene specific primer, FB09: 5'-ACGCCCTTTAATAACGAATATCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K034733-022-102-A03C-FB09
GGTAAATTAGTATTTAACAGAAACTCTTGGGACCCGAATGAATTAAATGGGTTAACGCCT
TTATGTAATGTCGCCGTCAACTTACAACAGTTTCTTCACGGCTTGTGCAATGAAAACGTG
CCGGAACTTGTAACGAACATTGTAGTTTGATTTTTGTAATCAACCATAGGAATAAATCAT
TTTTTCCGCATATAATTTATTTCCCTATTTCTATTCGTGACAAAACATTTGAGGATATAG
GAAATTAATAGCCTATAATATGTTTGACCATAAAAAGAATTTCTTCTTCTTGAACCATCA
TAAGAAAAATGTGTGTTTAGTCTATTGATCAGTTTTGTGTTCAAAAAAAAAAAAAAAATG
TCTATCGATCATTTAGGTGTAAAAAAAAAATTTAAAAAACTCGGGAAAAAAACTTTTTAT
TTGGAACACCCTTCATTGTCCTTTAAAAAAAAAAAAAAAAAAAAAACACTTTTTTTTTTT
TTTTTTTTTTTTTCGGAAAAGGTAAAAACCCACAAAAATTTTTGGGCAAAAATTGTTTTT
TTAAAAAAACAACCCAGGACTTTTTATTTGAAAAGGGTTTCTTGTCCGGAAAATCTACTG
GGATCACCAATGATTAAAAGGGTCAATAGGGAAAAAAAAAAAAAAAATTTACCATTTTTT
TTTCATTTCAAAAATGTAAATTCCCCCACCTTTTTTAAAAAAGAAAATTACTTTTTAAAA
AAACAACAAATTACAACCCTTCTTATTTATGGGCAAAACCAATAAAAAAATTTTTCTATT
TCGAAAATTTTTTTTTCAACGTGTC

Last Updated on 06.12.2018 16:11