Sequences from confirmation amplicon


Confirmation sequences for line 102F10 and hit At4g23420 are shown.
Line ID 102F10
Locus At4g23420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12226220
Primers used for PCR FT22 - 8474, show primer details
   
Sequence Name 57-K100940-0022-102-F10C-8409
GenBank Accession LN509357 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>57-K100940-0022-102-F10C-8409
CGGACTGAGCCTTTACAATTGAATTATCCTGAGTTCCCAATTGGTCATTTACAAATCTCA
GAGTTTGTGTTAATTTCTTCTTTCCTTACATCTTTTCCAGAAGAAAAAAAGCTGAAAGAT
GTGGTTTTTTGGATCGAAAGGAGCATCTGGATTCTCTTCTCGTTCCACAGCAGAGGAAGT
CACACATGGTGTTGATGGGACTGGTCTCACTGCCATTGTCACAGGTTTTCTTCTATCAAT
AGTTTCATGCCCTTTTGCGACTTGGGTTTGTCTAAAGTTGTAGACTTTTCTATAATTATG
AATTGATTTCATATGTAGGGTAGAGATTTTTTACTTTTATTGTTTAGACAATTGGAATTT
GATGTTTTTATGATCAGGGTCTGTTTTTGGTTCGGTCAACCCTAATAAATATATGGAGGT
CATCACCGTTTGTTGTTCTAAAGTTTGTCTTTTTTTTTTGCCAATAAGTAACAAGCTTTT
GCTCACAAGAATTGGATTGCTATGTTTGGTGAATTTTAAAGTACAAC
   
Sequence Name 57-K100947-0022-102-F10C-FT22
GenBank Accession LN509358 [GenBank]
Sequencing primer gene specific primer, FT22: 5'-TGGTCCCAAACATGTTGTACTTTA-3'
Sequence (A. th genome BLAST matches underlined)
>57-K100947-0022-102-F10C-FT22
CATCCATTCTTGTGAGCAAAGCTTGTTACTTATTGGCAAAAAAAAAGACAAACTTTAGAA
CAACAAACGGTGATGACCTCCATATATTTATTAGGGTTGACCGAACCAAAAACAGACCCT
GATCATAAAAACATCAAATTCCAATTGTCTAAACAATAAAAGTAAAAAATCTCTACCCTA
CATATGAAATCAATTCATAATTATAGAAAAGTCTACAACTTTAGACAAACCCAAGTCGCA
AAAGGGCATGAAACTATGGATAGAAGAAAACCTGTGACAATGGCAGTGAGACCAGTCCCA
TCAACACCATGTGTGACTTCCTCTGCTGTGGAACGAGAAGAGAATCCAGATGCTCCTTTC
GATCCAAAAAACCACATCTTTCAGCTTTTTTTCTTCTGGAAAAGATGTAAGGAAAGAAGA
AATTAACACAAACTCTGAGATTTGTAAATGACCAATTGGGAACTCAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
Primers used for PCR FT23 - 3144, show primer details
   
Sequence Name 51-K100940-0022-102-F10C-3144
GenBank Accession LN507436 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined)
>51-K100940-0022-102-F10C-3144
ATCCACTTCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTCGAA
GGATTTCGCTTAAGTTCTGATTTTAATAATTCATATGTGACAGAAAAGTGTGATTACTCT
AATTGGGAAGGATAAATAATGACGAGGAGAGAATCCTCGCTTCTCACGTGGAGATTTGAT
TAGAAGATTGCATCTCCAAAGTCAAATTAACAACCTTCAATAAAATAGCTCCACCAAAAA
CGTGAACAATTAAAGAAAGACCTCAGATGTTATAACAGTTACCAGAGAGCTAAAAAGATT
GACCTATTGC
   
Sequence Name 51-K100947-0022-102-F10C-FT23
GenBank Accession LN507437 [GenBank]
Sequencing primer gene specific primer, FT23: 5'-CCGCAATAGTTCAATCTTTTTAGC-3'
Sequence (A. th genome BLAST matches underlined)
>51-K100947-0022-102-F10C-FT23
TCTGAGTCTTTCTTAATTGTTCACGTTTTTGGTGGAGCTATTTTATTGAAGGTTGTTAAT
TTGACTTTGGAGATGCAATCTTCTAATCAAATCTCCACGTGAGAAGCGAGGATTCTCTCC
TCGTCATTATTTATCCTTCCCAATTAGAGTAATCACACTTTTCTGTCACATATGAATTAT
TAAAATCAGAACTTAAGCGAAATCCTTCGAACACTGATAGTTTAAACCGAAGGCGGGAAA
CGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGA
GGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATC

Last Updated on 06.12.2018 16:11