Sequences from confirmation amplicon


Confirmation sequences for line 103B06 and hit At2g30470 are shown.
Line ID 103B06
Locus At2g30470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12982705
Primers used for PCR ZZ16 - 8474, show primer details
   
Sequence Name 19-K020244-022-103-B06C-8409
GenBank Accession LN494570 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K020244-022-103-B06C-8409
AGATTTCCCGGCTAAGAATGGAGCTAAGCAGAAAATAAAAACCAGCACATTCCTACAGAA
ACCAGTTCATAGAAACCCCAACCTGCATGTCTCCAGCATTAGCTGCCTTCCTGGAACCCA
TGATTAGTTTTCCGCCAGGATCAACCCGACTGAAAGTTACTGTAGAGGTGAAAAACACAG
AAAACCAACAATAGTTTTAGAAAATGGTTCATGAAAATTTGATGTTAAAACCAGCAAATG
CTTGAAGCTTTAGCTAAGACATGAACTATATTAAAAGTACCTGTATCACCAGCCTGTAGC
ATCATGGACTGTATGCATGGAGTGACACCTTCTAAAACATACATTCTACTATTGTTATTG
GGCCAATATCTGAACTGGAACGTCCACTCCCTACCCCTCACATCTTGGATTTTCAAAGGA
ATGCCTTCGGATTGACTAATCGGAGGAAAATATGCCTATATACCAATGCAGACATCAGTC
ATAACTCTACAAAATATTTCAAGGACAAGAAAAGACATCACCATAGGAATGGGAAATTTA
CCTCTGCACAGGCTTTTGGAAGAACTAGACGACCAATGCGACCAGCATCACTGGCAC
   
Sequence Name 20-K020244-022-103-B06C-ZZ16
GenBank Accession LN495025 [GenBank]
Sequencing primer gene specific primer, ZZ16: 5'-TGAGAAAACTCTTAGTGCCAGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K020244-022-103-B06C-ZZ16
TCGCATTGGCAGTCTAGTTCTTCCAAAAGCCTGTGCAGAGGTAAATTTCCCATTCCTATG
GTGATGTCTTTTCTTGTCCTTGAAATATTTTGTAGAGTTATGACTGATGTCTGCATTGGT
ATATAGGCATATTTTCCTCCGATTAGTCAATCCGAAGGCATTCCTTTGAAAATCCAAGAT
GTGAGGGGTAGGGAGTGGACGTTCCAGTTCAGATATTGGCCCAATAACAATAGTAGAATG
TATGTTTTAGAAGGTGTCACTCCATGCATACAGTCCATGATGCTACAGGCTGGTGATACA
GGTACTTTTAATATAGTTCATGTCTTAGCTAAAGCTTCAAGCATTTGCTGGTTTTAACAT
CAAATTTTCATGAACCATTTTCTAAAACTATTGTTGGTTTTCTGTGTTTTTCACCTCTAC
AGTAACTTTCAGTCGGGTTGATCCTGGCGGAAAACTAATCATGGGTTCCAGGAAGGCAGC
TAATGCTGGAGACATGCAGGTTGGGGTTTCTATGAACTGGTTTCTGTAGGAATGTGCTGG
TTTTTATTTTCTGCTTAGCTCCATTCTTATCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
Primers used for PCR ZZ17 - 3144, show primer details
   
Sequence Name 21-K020244-022-103-B06C-3144
GenBank Accession LN495476 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K020244-022-103-B06C-3144
CGGATGACGCACACTCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCAT
TTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCACCTTGGGTTGTG
GGCTCACCAACGGAACATCAACTGAGGACACATCATCGTCTGGTGTAACAGAAAACCCAC
CCTCCATAAATGGTTCCTCGTGTATTTCACTAATACCGAAAGAGTTGAATGGTATGCCTG
AGAATTTGAACAGTGAGACTAACGGGGGCAGGATAGGTGATGATCCTACACGAGTTAAAG
AGAAGAAGAGAACTCGAACCATTGGTGCAAAAAATAAGAGACTTCTTTTGCATAGTGAAG
AATCTATGGAGCTGAGACTCACTTGGGAAGAAGCTCAGGACTTGCTTCGTCCCTCTCCTA
GTGTAAAG
   
Sequence Name 22-K020244-022-103-B06C-ZZ17
GenBank Accession LN495922 [GenBank]
Sequencing primer gene specific primer, ZZ17: 5'-CGATGGTAGGCTTTACACTAGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K020244-022-103-B06C-ZZ17
GAAGCAAGTCCTGAGCTTCTTCCCAAGTGAGTCTCAGCTCCATAGATTCTTCACTATGCA
AAAGAAGTCTCTTATTTTTTGCACCAATGGTTCGAGTTCTCTTCTTCTCTTTAACTCGTG
TAGGATCATCACCTATCCTGCCCCCGTTAGTCTCACTGTTCAAATTCTCAGGCATACCAT
TCAACTCTTTCGGTATTAGTGAAATACACGAGGAACCATTTATGGAGGGTGGGTTTTCTG
TTACACCAGACGATGATGTGTCCTCAGTTGATGTTCCGTTGGTGAGCCCACAACCCAAGG
TGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTT
ATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGG
AGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGA
TGCTCCTCGTGGGTGGGGG

Last Updated on 06.12.2018 16:11