Sequences from confirmation amplicon


Confirmation sequences for line 103B07 and hit At5g23690 are shown.
Line ID 103B07
Locus At5g23690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7987816
Primers used for PCR FT08 - 8474, show primer details
   
Sequence Name 57-K100897-0022-103-B07C-8409
GenBank Accession LN509355 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K100897-0022-103-B07C-8409
GCCATTTACAATTGAATATAACATATAAAATCGAATTAAGAAAGTAAAAACATATAACAG
AATCGAATAAATTGTAGAACTGGTTCATATAAATAGTTCACCTTCCATAAGCAGTGAGAC
TGGAGAGTACGAACAGGGGAAAAGGATCTGCAAGCAAAACCCACACTTGAAATCGCCATG
GAAATTGGAAAGGTTCAACATTTCATCATGGGTTTTCAAACTTTAATTCAGTGAATTGTG
ACTAGAACAGAGAGACTTGCAGAGAAGTTAGGGTTCTAGTTCCGGGAAGGGTTTTTGGCG
GATACCACGCGCCATCGCCACACGCCGCGGGACTAATTGATACTTGAAACTATTGTTGTT
TCAATGTTACTCTTTAGAACATCTTGCCGTTTTAATGACTGATCTAACAACTTTAATTAT
GTTGACCACTTTTATTTTAATAATTGTAAATGTAAACGCCAA
   
Sequence Name 57-K100905-0022-103-B07C-FT08
GenBank Accession LN509356 [GenBank]
Sequencing primer gene specific primer, FT08: 5'-TCATTGATTTATTGTTGGCGTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K100905-0022-103-B07C-FT08
TCACATAATTAAGTTGTTAGATCAGTCATTAAAACGGCAAGATGTTCTAAAGAGTAACAT
TGAAACAACAATAGTTTCAAGTATCAATTAGTCCCGCGGCGTGTGGCGATGGCGCGTGGT
ATCCGCCAAAAACCCTTCCCGGAACTAGAACCCTAACTTCTCTGCAAGTCTCTCTGTTCT
AGTCACAATTCACTGAATTAAAGTTTGAAAACCCATGATGAAATGTTGAACCTTTCCAAT
TTCCATGGCGATTTCAAGTGTGGGTTTTGCTTGCAGATCCTTTTCCCCTGTTCGTACTCT
CCAGTCTCACTGCTTATGGAAGGTGAACTATTTATATGAACCAGTTCTACAATTTATTCG
ATTCTGTTATATGTTTTTACTTTCTTAATTCGATTTTATATGTTATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTC
Primers used for PCR FT09 - 3144, show primer details
   
Sequence Name 58-K100897-0022-103-B07C-3144
GenBank Accession LN509646 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K100897-0022-103-B07C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTCATTCTGTTATATGTTGCT
GCTGCAATTGAGACAATGGATGAGTCAGATGGTTTTGCCACGGACAGTGATCGTCAAGAC
AAGGTTTCTGATGAACCAAGTAAGCTTAAGTCCATAATTGAACTGGAATTAGTCATCAAT
CCATTGTTCCTACTCATAATGTTAGGGTATCAACTCTGTTCGATTTTGGTTGATTTGGAA
AATGAAGGGGATCGTGAGTGGAAACAACTGAATTCGAAAGATCTTGGACTAAGCAGCTCA
ATGATTGCAAAATCCACAAGAAAAGTGCTTAATGGCCTTAAAAGCAAAGGTTGGT
   
Sequence Name 58-K100905-0022-103-B07C-FT09
GenBank Accession LN509647 [GenBank]
Sequencing primer gene specific primer, FT09: 5'-GACTTTGAAACTACCAACCTTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K100905-0022-103-B07C-FT09
TGGATTTTGCATCATTGAGCTGCTTAGTCCAGATCTTTCGAATTCAGTTGTTTCCACTCA
CGATCCCCTTCATTTTCCAAATCAACCAAAATCGAACAGAGTTGATACCCTAACATTATG
AGTAGGAACAATGGATTGATGACTAATTCCAGTTCAATTATGGACTTAAGCTTACTTGGT
TCATCAGAAACCTTGTCTTGACGATCACTGTCCGTGGCAAAACCATCTGACTCATCCATT
GTCTCAATTGCAGCAGCAACATATAACAGAATGAACCGAAGGCGGGAAACGACAATCTGA
TCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTT
GCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCA

Last Updated on 06.12.2018 16:11