Sequences from confirmation amplicon


Confirmation sequences for line 105D10 and hit At5g50430 are shown.
Line ID 105D10
Locus At5g50430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20535817
Primers used for PCR JM02 - 8474, show primer details
   
Sequence Name 13-K022051-022-105-D10C-8409
GenBank Accession LN491847 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K022051-022-105-D10C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATATATTACCTAACATGA
TATATTCACCATTACATATTACTGGTAATCCTGAAAACATGATACCAATAGTATCTCTGT
CACCATAGCAACACGCTATATGTACCTAAAGCCAGTATCCCCATAGCCAAACCTTCAATA
GAATAACTAGAAAGCAGAAAATAAAGAAAAGGAACATTAAATAGCCAGATAAGACTTACG
CCATTCAAGAATGTCATTTGGGGAAGGACGAGCAACAACATGCGAGACTGGTTCCTAGCA
ATCCAGAACAGAACATGTCAAAGATACTTCCAAAGTAACATTCATAAGAAAAAAACTTAT
CCTAGACCAAAAACCATGAAAACTAGCATAGAGCAAAGAAACCACATTTGATAAAAAAAA
AAATGAAAATCGAATGGGTAGAATGATACCAGTGACAGACAAATCAGGGATGGGAACAGA
ATAAGAAGAAAATAATACCTTGCAAAGCGCTCTGTATTCTTTTTGAAGACGCTTTATACA
AGCTTTTTCTGCCATCTAAATGCTTCAGGGAGTTCCAATAAACTGTAGTTCCTGTCAACC
AAAACAACGAATTGTAAACTGTAGAAAATATAATCCAACCTTTAAGTATGAAATGAATGA
CAGTAACATAAACCAAATTAAGGTCATCTTC
   
Sequence Name 14-K022051-022-105-D10C-JM02
GenBank Accession LN492316 [GenBank]
Sequencing primer gene specific primer, JM02: 5'-TGACTTAAGCAGGATGGTTTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K022051-022-105-D10C-JM02
GAAGATGACCTTAATTTGGTTTATGTTACTGTCATTCATTTCATACTTAAAGGTTGGATT
ATATTTTCTACAGTTTACAATTCGTTGTTTTGGTTGACAGGAACTACAGTTTATTGGAAC
TCCCTGAAGCATTTAGATGGCAGAAAAAGCTTGTATAAAGCGTCTTCAAAAAGAATACAG
AGCGCTTTGCAAGGTATTATTTTCTTCTTATTCTGTTCCCATCCTTGATTTGTCTGTCAC
TGGTATCATTCTACCCATTCGATTTTCATTTTTTTTTTTATCAAATGGGGTTTCTTTGCT
CTATGCTAGTTTTCAGGGTTTTTGGTCTAGGAAAAGTTTTTTTCTTATGAATGTTACTTT
GGAAGTATCTTTGACATGTTCTGTTCTGGATTGCTAGGAACCAGTCTCGCAGGTTGTTGC
TCGTCCTTCCCCAAATGACATTCTTGAAGGGCGTAAGTCTTATCTGGCTATTTAAGGTTC
CTTTTCTTTATTTTCGGCTTTCTAGTTATTCTATTGAAGGTTTGGCTATGGGGATACTGG
CTTTAGGTACATATAGCGTGTTGCTATGGTGACAAAAATACTATTGGTATCATGTTTTCA
GGATTACCAGTAATATGTA

Last Updated on 06.12.2018 16:11