Sequences from confirmation amplicon


Confirmation sequences for line 107A11 and hit At5g43170 are shown.
Line ID 107A11
Locus At5g43170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17331923
Primers used for PCR KS52 - 8474, show primer details
   
Sequence Name 26-K033098-022-107-A11C-KS52
GenBank Accession LN497815 [GenBank]
Sequencing primer gene specific primer, KS52: 5'-GGATGGTGTCGATTTATCATTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K033098-022-107-A11C-KS52
GCTCGCTTTATGACCGCCGAGAGCTTGGTAAGACGAAAACGTCTTGTAACAAACGCCACA
CTTATAACTCGGCTTCTCCGCAACCGTCACAGAGTCAAGATCGCCGCCATCCCGAGCAAG
AAGCATGAGACAGAAAGCTAGATACTCTTCCTCAGTGAGACGGTGGTTATGATGAAGATC
AGATCTCGAACGTTTGGATCGTTTCTTACATTTGGTCCACTGCTCAACGCCATTGAATCT
TAAGGGATCCTCGACCAATCTTGGTGAATTAAGAGCTTCAAGCGCCATTGCTATAGAAAA
TTTAAAACCTAATTTGAGAGCAGAGAGATATATAGAAGAAGGTGAAGTATGGAGAAGAAT
CTTGAGAAGAGAGGAAGTTGCGTGAGAAAGAAGAGTGAGAGTGGGTGGCCAAATTGGCCA
TTCTAATTATTTTAAGTATTTAGTTGAAACGAAATATGTGCTGAGCAAGTTGTTTGTTGT
AAGTTGTAACGAAAGTGAATGTATAATTAAAATATTATTTCAACATTTGGTGAGAGTGGA
ATATATTAATTTTGGCACGTTTTGTGGGGGAGAGAAATGTTACACAAGTCGGGCAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGT
   
Sequence Name 74-K033098-022-107-A11C-8409
GenBank Accession LN513004 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K033098-022-107-A11C-8409
GTACATTTCTCTCCCCCCAAAACGTGCCAAAATTAATATATTCCACTCTCACCAAATGTT
GAAATAATATTTTAATTATACATTCACTTTCGTTACAACTTACAACAAACAACTTGCTCA
GCACATATTTCGTTTCAACTAAATACTTAAAATAATTAGAATGGCCAATTTGGCCACCCA
CTCTCACTCTTCTTTCTCACGCAACTTCCTCTCTTCTCAAGATTCTTCTCCATACTTCAC
CTTCTTCTATATATCTCTCTGCTCTCAAATTAGGTTTTAAATTTTCTATAGCAATGGCGC
TTGAAGCTCTTAATTCACCAAGATTGGTCGAGGATCCCTTAAGATTCAATGGCGTTGAGC
AGTGGACCAAATGTAAGAAACGATCCAAACGTTCGAGATCTGATCTTCATCATAACCACC
GTCTCACTGAGGAAGAGTATCTAGCTTTCTGTCTCATGCTTCTTGCTCGGGATGGCGGCG
ATCTTGACTCTGTGACGGTTGCGGAGAAGCCGAGTTATAAGTGTGGCGTTTGTTACAAGA
CGTTTTCGTCTTACCAAGCTCTCGGCGGTCATAAAGCGAGCCACCGGAGCTTATACGGTG
GTGGAGAGAAT

Last Updated on 06.12.2018 16:11