Sequences from confirmation amplicon


Confirmation sequences for line 109C11 and hit At1g66230 are shown.
Line ID 109C11
Locus At1g66230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24677971
Primers used for PCR M20a - 8474, show primer details
   
Sequence Name 27-K014705-022-109-C11C-8409
GenBank Accession LN498155 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K014705-022-109-C11C-8409
CTGTAGATTTCCCGGACATGAAGCCATTTACAATTGAAACAGAGTCTTTAGACCTTATCT
GCAGCAATTCAACAATGTCTTCATCCACGTCCACATCTTCGCATTCTTCTAATGATTCGA
GTTTCTTGAAGGATTTGCAGTTCCCGGAGTTCGAGTGGTCCGACTATGGTAATAGTAATA
ATGATAATAATAATGGTGTGGACAACATTATAGAGAACAATATGATGAGCCTGTGGGAAA
TTAGTGACTTTAGCAGTTTGGATTTGCTGCTTAATGATGAGAGTTCTTCCACTTTTGGGT
TGTTTTGAATTCATTCTTAAGAATATGGTTTCTTTTGTAAAAGAATGGAAACTATGGGGT
GGGGGAAACAGAAAAACCTAAATACCCTTCGTTACTCTAGTTTTTGAGGGATTTGAGAGA
ACGTTTTTTTACTTGTGAGAAATTTAGAAACAAAAATAAAGTTTATTTATTTTGTACAGA
CAAAAAGAGTTAAGAGAGAAATGTGTCTTTGGCCCATTTCTTTTTTTGCTTTGTATATTG
GGTTCCATA
   
Sequence Name 28-K014705-022-109-C11C-M20a
GenBank Accession LN498621 [GenBank]
Sequencing primer gene specific primer, M20a: 5'-CGAGGAAGTATGGAACCCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K014705-022-109-C11C-M20a
ATACAAAGCAAAAAAAGAAATGGACCAAAGACACATTTCTCTCTTAACTCTTTTTGTCTG
TACAAAATAAATAAACTTTATTTTTGTTTCTAAATTTCTCACAAGTAAAAAAACGTTCTC
TCAAATCCCTCAAAAACTAGAGTAACGAAGGGTATTTAGGTTTTTCTGTTTCCCCCACCC
CATAGTTTCCATTCTTTTACAAAAGAAACCATATTCTTAAGAATGAATTCAAAACAACCC
AAAAGTGGAAGAACTCTCATCATTAAGCAGCAAATCCAAACTGCTAAAGTCACTAATTTC
CCACAGGCTCATCATATTGTTCTCTATAATGTTGTCCACACCATTATTATTATCATTATT
ACTATTACCATAGTCGGACCACTCGAACTCCGGGAACTGCAAATCCTTCAAGAAACTCGA
ATCATTAGAAGAATGCGAAGATGTGGACGTGGATGAAGACATTGTTGAATTGCTGCAGAT
AAGGTCTAAAGACTCTGTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACG
ACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGA
AATCTTTATTTCGACGTGTCTACATTCACG

Last Updated on 06.12.2018 16:11