Sequences from confirmation amplicon


Confirmation sequences for line 110D02 and hit At5g26030 are shown.
Line ID 110D02
Locus At5g26030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9097395
Primers used for PCR MR25 - 3144, show primer details
   
Sequence Name 43-K017390-022-110-D02C-MR25
GenBank Accession LN504666 [GenBank]
Sequencing primer gene specific primer, MR25: 5'-TTGTTGCAGAAGATAAGATTGGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K017390-022-110-D02C-mr25
CCTGAAACTCTTAACGATGTTCAACCTTTCTTGTATAATCTCTTTGCTGATCCGGTGAGA
ATCTTTTGACTTTGTGTGTTTGTGATTGATTTGTGTTGAAAGTGGGATGCTGAAATGGTT
TTGTTGTTGTTGTTGAAGGATATTATAAGGCTTCCTAGACCATTTCAGTTTCTTCAAGGG
ACTATAGCTAAGTTTATATCTGTTGTTCGTGCTCCGAAATCTAAAGAAGGGTATGCTGCT
ATTGGTGGTGGCTCTCCTTTGCGTAAAATAACTGATGAGCAAGCGGATGCTATTAAGATG
TCTTTGCAAGCGAAGAACATTGCTGCTAATGTCAAACACTGATAGTTTAAACCGAAGGCG
GGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTAT
ATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAG
ATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATG
CTCCTCGTGGGTGGGGG
   
Sequence Name 44-K017390-022-110-D02C-3144
GenBank Accession LN505025 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K017390-022-110-D02C-3144
GGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATT
TCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAA
CTATCAGTGTTTGACATTAGCAGCAATGTTCTTCGCTTGCAAAGACATCTTAATAGCATC
CGCTTGCTCATCAGTTATTTTACGCAAAGGAGAGCCACCACCAATAGCAGCATACCCTTC
TTTAGATTTCGGAGCACGAACAACAGATATAAACTTAGCTATAGTCCCTTGAAGAAACTG
AAATGGTCTAGGAAGCCTTATAATATCCTTCAACAACAACAACAAAACCATTTCAGCATC
CCACTTTCAACACAAATCAATCACAAACACACAAAGTCAAAAGATTCTCACCGGATCAGC
AAAGAGATTATACAAGAAAGGTTGAACATCGTTAAGAGTTTCAGGACCACCTAAATTCAA
AAGCAAGACACCAATCTTATCTT
Primers used for PCR FI62 - 8474, show primer details
   
Sequence Name 21-K028244-022-110-D02C-8409
GenBank Accession LN495529 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K028244-022-110-D02C-8409
TAGATTTCCCGGACATTAAGCGGTATTGGTATCCGTTCACTGAGGAGGCTGTTCAGCAGG
TGTGATTTGCTCTCAGTATTGATGGAGTTTCTTATGTTTGTGTCATAGTCATGTTTATCT
TAACAAGCTAATTTCTTTTAATCAGATAAAGAAGGACAAAATTACTAGACTTGTTGTACT
GCCATTGTATCCTCAGTATTCTATCTCTACAACGGGTTCAAGCATACGCGTTCTCCAAGA
TTTATTCAGGTTTGTGCAAGCTACAGAGCATATTCGTAGTTTATTGGTTGTTTACTTTTA
AATGATTTCTTAGTTGCACGAGCGGTACTTAACATGTATCATCATTCAGGAAAGATCCGT
ACCTAGCTGGAGTGCCGGTAGCTATTATAAAGTCCTGGTACCAAAGGCGAGGCTATGTCA
ATTCTATGGCTGACCTCATTGAGAAGGAGCTTCAAACTTTCTCTGATCCTAAGGAGGTAT
AGTTATATCCCCCCCCCCCTCTGTTTTTTCCTCTTCATTGCACATTTCTAGTCTGAACAC
TGGTTAAATTGGTCCATTGTTTTGTGATTTAGGTTATGATATTCTTCAGTGCCCATGGTG
TTCCGGTCAGCTACGTA
   
Sequence Name 22-K028244-022-110-D02C-FI62
GenBank Accession LN495975 [GenBank]
Sequencing primer gene specific primer, FI62: 5'-GTACGGATCTCCAGCATTCTCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K028244-022-110-D02C-FI62
CCGGAACACCATGGGCACTGAAGAATATCATAACCTAAATCACAAAACAATGGACCAATT
TAACCAGTGTTCAGACTAGAAATGTGCAATGAAGAGGAAAAAACAGAGGGGGGGGGGGAT
ATAACTATCCCTCCTTAGGATCAAAAAAAGTTTGAACCTCCTTCTCAATGAGGTCACCCA
TAAAATTGACATACCCCCCCCTTTGGTACCAGGACTTTATAATACCTACCGGCACTCCAC
CTAGGTACGGATCTTTCCTGAATGATGATACATGTTAAGTACCCCTCGTGCAACTAAAAA
ATCTTTTAAAAGTAAACAACCAATAAACTCCGAATATGCTCTGTAGCTTGCACAAACCTG
AATAAATCTTGGAAAACCCGTATGCTTGAACCCTTTGTAAAAATAAAATACTGAGGATAC
AATGGCAGTACAACAAGTCTAGAAATTTTGCCCTTCTTTATCTGATTAAAAAAAATTACC
TTGTTAAAATAAACTTGACTTTGACCCAAACTTAAGAAACTCCTTCAATACTGAGAGCAA
ATCACACCTGCTGAACAGCCTCCTCAGTGAACGGATACCAATACCGCTAAATGTCCGGGA
AAATCTACTTGGATCACCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCAGCGTTTTTATAAAATGA

Last Updated on 06.12.2018 16:11