Sequences from confirmation amplicon


Confirmation sequences for line 111E03 and hit At1g14440 are shown.
Line ID 111E03
Locus At1g14440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4940392
Primers used for PCR 28jd - 8474, show primer details
   
Sequence Name 74-K113077-0022-111-E03C-28jd
GenBank Accession LN513105 [GenBank]
Sequencing primer gene specific primer, 28jd: 5'-AATTGGGTCTTCTTCAAAAACCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K113077-0022-111-E03C-28jd
TTGGGGACGAGGAGAGACTAGCTAAGAAGAAAACAGACAATTGGAGGAAACTATTAGTAA
CTAATACCAAAATGATTAAACTATTAAAGGTGAATACTTTCTTGATTAAAATCTTAAGAA
GTTTCCACTTTTTTAGAAACAACTCACGGAAAGCATTTTTCAAATAATTTTACTAAAAGA
AACAACCAAAATTTACAAAACCCAAAATTATAGGACAAAACGAAAGAATACAAAAAAATG
GTAATTAAGGAAAATAAAACTCTTATTGCAAGCCTGCGCAAACAAAAGCTGACAAACGCA
CTTCGAAAGAAGTCTACATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 47-K113075-0022-111-E03C-8409
GenBank Accession LN506248 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K113075-0022-111-E03C-8409
TTGAATTATCCATGTAGACTTCTTTCGAAGTGCGTTTGTCAGCCTTTGTTTGCGCAGGCT
TGCAATAAGAGTTTTATTTTCCTTAATTACCATTTTTTTGTATTCTTTCGTTTTGTCCTA
TAATTTTGGGTTTTGTAAATTTTGGTTGTTTCTTTTAGTAAAATTATTTGAAAAATGCTT
TCCGTGAGTTGTTTCTAAAAAAGTGGAAACTTCTTAAGATTTTAATCAAGAAAGTATTCA
CCTTTAATAGTTTAATCATTTTGGTATTAGTTACTAATATTTTCCTCCAATTGTCTTGTT
TTCTTCTTAGCTAGTCTCTCCTCTGTCCCCAAACCAAGAAACAATTTGCAAAGAGAAAGC
AAAAGGTTTTTGAAGAGAACCCAAT

Last Updated on 06.12.2018 16:11